Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys

被引:90
作者
Hauet, T
Goujon, JM
Baumert, H
Petit, I
Carretier, M
Eugene, M
Vandewalle, A
机构
[1] Univ Paris 07, INSERM, U478, Inst Fed Rech 02, F-75870 Paris 18, France
[2] Ctr Hosp & Univ, Fac Med, INRA, Dept Genet Anim,Unite Transplantat Expt, Poitiers, France
[3] Hop St Louis, Lab Resonance Magnet Nucl & Explorat Fonct, Paris, France
关键词
transplantation; organ storage; MHC class II; reperfusion injury; delayed graft function; cold organ injury; interstitial fibrosis; renal allograft rejection;
D O I
10.1046/j.1523-1755.2002.00473.x
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Background. The conditions of storage of donor kidney may influence the deleterious consequences of ischemia/reperfusion injury (IRI) on delayed graft function. Since polyethylene glycol (PEG) can protect renal tubule cells against cold injury, we tested the effects of adding PEG 20 kD to ice-cold preservation solutions on the IRI of autotransplanted pig kidneys. Methods. The pigs' left kidneys were removed, cold-flushed with University of Wisconsin (UW) or simplified high K+ or high Na+ solutions with or without 30 g/L PEG 20M and stored for 48 hours at 4degreesC. The kidneys were then autotransplanted and the contralateral kidneys were removed. Kidney biopsies were then performed and renal function parameters were analyzed over 8 to 12 weeks following surgery. Results. The kidneys cold-flushed with PEG-supplemented solutions on day 7 post-transplantation were better preserved and exhibited less marked nuclear tubular cell damage than the kidneys cold-flushed with the UW solution alone. PEG also almost completely inhibited the overexpression of major histocompatibility complex class II that was detected in epithelial tubule cells from kidneys cold-flushed with the UW solution. PEG also significantly reduced the number of CD4(+) T lymphocytes and limited the infiltration of macrophages/monocytes and the progression of interstitial fibrosis in the 8- to 12-week post-transplanted kidneys. Moreover, pigs autotransplanted with kidneys flushed with PEG-supplemented solutions had the best renal function and the lowest levels of proteinuria. Conclusions. These findings indicate that PEG inhibits the early inflammatory response due to IRI, improves renal function, and may prevent the progression of interstitial fibrosis in the long-term autotransplanted pig kidney.
引用
收藏
页码:654 / 667
页数:14
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