Human Muse cells reduce myocardial infarct size and improve cardiac function without causing arrythmias in a swine model of acute myocardial infarction

被引:14
作者
Yamada, Yoshihisa [1 ]
Minatoguchi, Shingo [1 ]
Baba, Shinya [2 ]
Shibata, Sanae [3 ]
Takashima, Satoshi [3 ]
Wakao, Shohei [4 ]
Okura, Hiroyuki [1 ]
Dezawa, Mari [4 ]
Minatoguchi, Shinya [2 ,5 ]
机构
[1] Gifu Univ, Dept Cardiol, Grad Sch Med, Gifu, Japan
[2] Gifu Municipal Hosp, Gifu, Japan
[3] Gifu Univ, Anim Teaching Hosp Anesthesiol, Fac Appl Biol Sci, Gifu, Japan
[4] Tohoku Univ, Dept Stem Cell Biol & Histol, Grad Sch Med, Sendai, Miyagi, Japan
[5] Gifu Univ, Dept Circulatory & Resp Adv Med, Grad Sch Med, Gifu, Japan
来源
PLOS ONE | 2022年 / 17卷 / 03期
关键词
PLURIPOTENT STEM-CELLS; REPAIR; HEART;
D O I
10.1371/journal.pone.0265347
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background We recently reported that multilineage-differentiating stress enduring (Muse) cells intravenously administered after acute myocardial infarction (AMI), selectively engrafted to the infarct area, spontaneously differentiated into cardiomyocytes and vessels, reduced the infarct size, improved the left ventricular (LV) function and remodeling in rabbits. We aimed to clarify the efficiency of Muse cells in a larger animal AMI model of mini-pigs using a semiclinical grade human Muse cell product. Method and result Mini-pigs underwent 30 min of coronary artery occlusion followed by 2 weeks of reperfusion. Semi-clinical grade human Muse cell product (1x10(7), Muse group, n = 5) or saline (Vehicle group, n = 7) were intravenously administered at 24 h after reperfusion. The infarct size, LV function and remodeling were evaluated by echocardiography. Arrhythmias were evaluated by an implantable loop recorder. The infarct size was significantly smaller in the Muse group (10.5 +/- 3.3%) than in the Vehicle group (21.0 +/- 2.0%). Both the LV ejection fraction and fractional shortening were significantly greater in the Muse group than in the Vehicle group. The LV end-systolic and end-diastolic dimensions were significantly smaller in the Muse group than in the Vehicle group. Human Muse cells homed into the infarct border area and expressed cardiac troponin I and vascular endothelial CD31. No arrhythmias and no blood test abnormality were observed. Conclusion Muse cell product might be promising for AMI therapy based on the efficiency and safety in a mini-pig AMI.
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页数:12
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