Genome sequence of the recombinant protein production host Pichia pastoris

被引：387

作者：

De Schutter, Kristof ^{[1
,2
]}

Lin, Yao-Cheng ^{[3
,4
]}

Tiels, Petra ^{[1
,5
]}

Van Hecke, Annelies ^{[1
,5
]}

Glinka, Sascha ^{[6
]}

Weber-Lehmann, Jacqueline ^{[6
]}

Rouze, Pierre ^{[3
,4
]}

Van de Peer, Yves ^{[3
,4
]}

Callewaert, Nico ^{[1
,5
]}

机构：

[1] VIB, Dept Mol Biomed Res, Unit Mol Glycobiol, Ghent, Belgium

[2] Univ Ghent, Dept Biomed Mol Biol, B-9000 Ghent, Belgium

[3] VIB, Dept Plant Syst Biol, Ghent, Belgium

[4] Univ Ghent, Dept Plant Biotechnol & Genet, B-9000 Ghent, Belgium

[5] Univ Ghent, Dept Biochem & Microbiol, L ProBE, Unit Mol Glycobiol, B-9000 Ghent, Belgium

[6] Eurofins MWG Operon, Ebersberg, Germany

来源：

NATURE BIOTECHNOLOGY | 2009年 / 27卷 / 06期

关键词：

TRANSFER-RNA GENES; EXPRESSION; YEAST; GLYCOSYLATION; PREDICTION; IDENTIFICATION; ALGORITHM; ACCURACY; PROMOTER; FEATURES;

D O I：

10.1038/nbt.1544

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation. P. pastoris strains capable of human-type N-glycosylation are now available, which increases the utility of this organism for biopharmaceutical production. Despite its biotechnological importance, relatively few genetic tools or engineered strains have been generated for P. pastoris. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris. We also provide manually curated annotation for its 5,313 protein-coding genes.

引用

页码：561 / +

页数：9

共 55 条

[1] Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris:: production of complex humanized glycoproteins with terminal galactose [J].

Bobrowicz, P ;

Davidson, RC ;

Li, HJ ;

Potgieter, TI ;

Nett, JH ;

Hamilton, SR ;

Stadheim, TA ;

Miele, RG ;

Bobrowicz, B ;

Mitchell, T ;

Rausch, S ;

Renfer, E ;

Wildt, S .

GLYCOBIOLOGY, 2004, 14 (09) :757-766

[2]

Bretthauer RK, 1999, BIOTECHNOL APPL BIOC, V30, P193

[3] Recombinant protein expression in Pichia pastoris [J].

Cregg, JM ;

Cereghino, JL ;

Shi, JY ;

Higgins, DR .

MOLECULAR BIOTECHNOLOGY, 2000, 16 (01) :23-52

[4] PICHIA-PASTORIS AS A HOST SYSTEM FOR TRANSFORMATIONS [J].

CREGG, JM ;

BARRINGER, KJ ;

HESSLER, AY ;

MADDEN, KR .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3376-3385

[5] β-1,2- and α-1,2-linked oligomannosides mediate adherence of Candida albicans blastospores to human enterocytes in vitro [J].

Dalle, F ;

Jouault, T ;

Trinel, PA ;

Esnault, J ;

Mallet, JM ;

d'Athis, P ;

Poulain, D ;

Bonnin, A .

INFECTION AND IMMUNITY, 2003, 71 (12) :7061-7068

[6] MUSCLE: multiple sequence alignment with high accuracy and high throughput [J].

Edgar, RC .

NUCLEIC ACIDS RESEARCH, 2004, 32 (05) :1792-1797

[7] Locating proteins in the cell using TargetP, SignalP and related tools [J].

Emanuelsson, Olof ;

Brunak, Soren ;

von Heijne, Gunnar ;

Nielsen, Henrik .

NATURE PROTOCOLS, 2007, 2 (04) :953-971

[8] An efficient algorithm for large-scale detection of protein families [J].

Enright, AJ ;

Van Dongen, S ;

Ouzounis, CA .

NUCLEIC ACIDS RESEARCH, 2002, 30 (07) :1575-1584

[9]

Felsenstein J, 1996, METHOD ENZYMOL, V266, P418

[10] The Pfam protein families database [J].

Finn, Robert D. ;

Tate, John ;

Mistry, Jaina ;

Coggill, Penny C. ;

Sammut, Stephen John ;

Hotz, Hans-Rudolf ;

Ceric, Goran ;

Forslund, Kristoffer ;

Eddy, Sean R. ;

Sonnhammer, Erik L. L. ;

Bateman, Alex .

NUCLEIC ACIDS RESEARCH, 2008, 36 :D281-D288

← 1 2 3 4 5 6 →