Detection of colon cancer by laser induced fluorescence and Raman spectroscopy

Laser-induced fluorescence and Raman spectroscopy of serum for detect colon cancer was investigated in this paper. The difference to serum spectrum was discovered. Three Raman peaks were consistently observed from normal blood serum emission using 488.0nm and 514.5nm excitation of an Ar-ion laser. While no Raman peak or slight Raman peaks were detected from colon cancer's cases. In addition, the red shift of fluorescence peak and decrease of fluorescence intensity are founded after samples radiated by laser. 65 colon cancer cases are investigated in this paper. Through three parameters we obtained an accuracy of 83.5% compared to clinical diagnosis. These results have important reference values to explore the method of Raman spectrum and for diagnosis of cancer.