Cronobacter spp. isolated from aquatic products in China: Incidence, antibiotic resistance, molecular characteristic and CRISPR diversity

被引：20

作者：

Li, Chengsi ^{[1
]}

Zeng, Haiyan ^{[1
]}

Zhang, Jumei ^{[1
]}

Luo, Dandan ^{[1
]}

Chen, Moutong ^{[1
]}

Lei, Tao ^{[1
]}

Yang, Xiaojuan ^{[1
]}

Wu, Haoming ^{[1
]}

Cai, Shuzhen ^{[1
]}

Ye, Yingwang ^{[1
]}

Ding, Yu ^{[2
]}

Wang, Juan ^{[3
]}

Wu, Qingping ^{[1
]}

机构：

[1] Guangdong Acad Sci, Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Safety & Hlth, State Key Lab Appl Microbiol Southern China, Guangzhou 510070, Peoples R China

[2] Jinan Univ, Dept Food Sci & Technol, Guangzhou, Peoples R China

[3] South China Agr Univ, Coll Food Sci, Guangzhou, Peoples R China

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2020年 / 335卷

基金：

中国国家自然科学基金;

关键词：

Cronobacter; Prevalence; Antibiotic resistance; Meat and meat products; Multilocus sequence typing; CRISPR; ANTIGEN GENE CLUSTERS; ENTEROBACTER-SAKAZAKII; INFECTIONS; PREVALENCE; VEGETABLES;

D O I：

10.1016/j.ijfoodmicro.2020.108857

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Cronobacter species (Cronobacter spp.) are important foodborne pathogens that can infect and cause serious life-threatening diseases in infants and immunocompromised elderly. This study aimed to acquire data on Cronobacter spp. contamination of aquatic products in China from 2011 to 2016. In total, 800 aquatic products were tested, and the overall contamination rate for Cronobacter spp. was 3.9% (31/800). The average contamination level of the positive samples was 2.05 MPN/g. Four species and nine serotypes were identified among 33 isolates, of which the C. sakazakii serogroup O1 (n = 9) was the primary serotype. The majority of Cronobacter spp. strains harbored highest resistance against cephalothin (84.8%), followed by tetracycline (6.1%), trimethoprim/sulfameth-oxazole (3.0%) and chloramphenicol (3.0%). Two isolates were resistant to three antibiotics. In total, 26 sequence types and 33 CRISPR types (including 6 new STs and 26 new CTs) were identified, which indicates the extremely high diversity of Cronobacter spp. in aquatic products. Pathogenic C. sakazakii ST4, ST1, and C. malonaticus ST7 were also observed. Overall, this large-scale study revealed the relatively low prevalence and high genetic diversity of Cronobacter spp. in aquatic products in China, and the findings provide valuable information that can guide the establishment of effective measures for the control and precaution of Cronobacter spp. in aquatic products during production processes.

引用

页数：5

共 35 条

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