Quantum dots-based immunofluorescence technology for the quantitative determination of HER2 expression in breast cancer

被引：177
作者：
Chen, Chuang [1 ,2 ,3 ]
Peng, Jun [4 ,5 ]
Xia, He-Shun [6 ]
Yang, Gui-Fang [7 ]
Wu, Qjong-Shui [8 ]
Chen, Liang-Dong [1 ,2 ,3 ]
Zeng, Li-Bo [8 ]
Zhang, Zhi-Ling [4 ,5 ]
Pang, Dai-Wen [1 ,4 ,5 ]
Li, Yan [1 ,2 ,3 ]
机构：
[1] Wuhan Univ, Zhongnan Hosp, Dept Oncol, Wuhan 430071, Peoples R China
[2] Hubei Canc Clin Study Ctr, Wuhan 430071, Peoples R China
[3] Hubei Key Lab Tumor Biol Behav, Wuhan 430071, Peoples R China
[4] Wuhan Univ, State Key Lab Virol, Wuhan 430071, Peoples R China
[5] Wuhan Univ, Minist Educ, Key Lab Analyt Chem Biol & Med, Coll Chem & Mol Sci, Wuhan 430071, Peoples R China
[6] Hubei Canc Hosp, Dept Pathol, Wuhan 430079, Hubei, Peoples R China
[7] Wuhan Univ, Zhongnan Hosp, Dept Pathol, Wuhan 430071, Peoples R China
[8] Wuhan Univ, Elect Informat Sch, Sci Instrument Engn Res Ctr, Wuhan 430072, Peoples R China
来源：
BIOMATERIALS | 2009年 / 30卷 / 15期
基金：
中国国家自然科学基金;
关键词：
Quantum dots; Breast cancer; HER2; Immunohistochemistry; Fluorescence in situ hybridization; TIME; TRASTUZUMAB; BIOMARKERS; MORTALITY; ONCOGENE; THERAPY; PROBES; TUMORS;
D O I：
10.1016/j.biomaterials.2009.02.010
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
HER2 detection is important for breast cancer (BC) treatment and prognosis, but the detection methods currently used have some disadvantages. Quantum dots (QDs)-based probes provide a potentially important new method for HER2 detection in clinical practice. This potential is examined in this paper. A QDs HER2 probe kit and QDs image acquisition and analysis software were developed and applied to 94 clinical samples of BC. Compared to conventional immunohistochemistry techniques, this method provided a superior accurate and sensitive method for the detection of HER2 in clinical breast cancer diagnosis. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2912 / 2918
页数：7
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