Changes in Abundance of Oral Microbiota Associated with Oral Cancer

被引:275
作者
Schmidt, Brian L. [1 ,2 ]
Kuczynski, Justin [3 ]
Bhattacharya, Aditi [4 ]
Huey, Bing [4 ]
Corby, Patricia M. [1 ]
Queiroz, Erica L. S. [1 ]
Nightingale, Kira [1 ]
Kerr, A. Ross [5 ]
DeLacure, Mark D. [6 ,7 ]
Veeramachaneni, Ratna [4 ]
Olshen, Adam B. [4 ]
Albertson, Donna G. [4 ]
机构
[1] NYU, Coll Dent, Bluestone Ctr Clin Res, New York, NY 10012 USA
[2] NYU, Coll Dent, Dept Oral & Maxillofacial Surg, New York, NY USA
[3] Second Genome, Bioinformat Dept, San Bruno, CA USA
[4] Univ Calif San Francisco, Helen Diller Family Comprehens Canc Ctr, San Francisco, CA 94143 USA
[5] NYU, Coll Dent, Dept Oral & Maxillofacial Pathol Radiol & Med, New York, NY USA
[6] NYU, Dept Otolaryngol, New York, NY 10016 USA
[7] NYU, Dept Plast Surg, New York, NY USA
基金
美国国家卫生研究院;
关键词
FUSOBACTERIUM-NUCLEATUM; BACTERIA PRESENT; NECK-CANCER; DIVERSITY; HEAD; PERIODONTITIS; MICROFLORA; EXPRESSION; CARCINOMA; SEQUENCES;
D O I
10.1371/journal.pone.0098741
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Individual bacteria and shifts in the composition of the microbiome have been associated with human diseases including cancer. To investigate changes in the microbiome associated with oral cancers, we profiled cancers and anatomically matched contralateral normal tissue from the same patient by sequencing 16S rDNA hypervariable region amplicons. In cancer samples from both a discovery and a subsequent confirmation cohort, abundance of Firmicutes (especially Streptococcus) and Actinobacteria (especially Rothia) was significantly decreased relative to contralateral normal samples from the same patient. Significant decreases in abundance of these phyla were observed for pre-cancers, but not when comparing samples from contralateral sites (tongue and floor of mouth) from healthy individuals. Weighted UniFrac principal coordinates analysis based on 12 taxa separated most cancers from other samples with greatest separation of node positive cases. These studies begin to develop a framework for exploiting the oral microbiome for monitoring oral cancer development, progression and recurrence.
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页数:12
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