Effect of matrine on transforming growth factor β1 and hepatocyte growth factor in rat liver fibrosis model

被引:27
|
作者
Yu, Jian-Lan [1 ]
Li, Jun-Hua [2 ]
Cheng, Rong-Gui [1 ]
Ma, Yan-Mei [3 ]
Wang, Xiao-Juan [3 ]
Liu, Jing-Chun [4 ]
机构
[1] Changzhi Med Sch, Heji Hosp, Changzhi 046011, Peoples R China
[2] Tumor Hosp Shanxi Prov, Taiyuan 030013, Peoples R China
[3] Shanxi Med Sch, Taiyuan 030001, Peoples R China
[4] Shanxi Med Sch, Clin Hosp 1, Taiyuan 030001, Peoples R China
关键词
Liver fibrosis; Matrine; Transforming growth factor beta 1; Hepatocyte growth factor; STIFFNESS MEASUREMENTS; PERFORMANCE;
D O I
10.1016/S1995-7645(14)60062-6
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Objective: To observe the preventive and control effect of matrine on transforming growth factor (TGF-beta 1) and hepatocyte growth factor (HGF) of liver fibrosis tissue in rats. Methods: A total of 48 SD rats were randomly divided into A, B, C, D groups with 12 in each, group A as the normal control group and groups B, C, D as liver fibrosis models using composite modulus method with carbon tetrachloride (CCL4). Group B was the model group, group C adopted gamma-interferon lavage therapy in the second day of modeling, and group D adopted marine lavage treatment, at 4 and 8 weeks after treatment. Six rats were executed for detection of TGF-beta 1 and HGF, liver tissue histology and comparison fibrosis degree changes of rat liver tissue between groups. Results: Groups B, C, D showed a more significantly increased TGF-beta 1 at each time point compared with group A (P<0.05); Group B showed a more significantly increased TGF-beta 1 than groups C and D at weeks 4 and 8 (P<0.05); group D showed a lowest level of TGF-beta 1, followed by groups C and B. HGF of group B decreased more significantly than A group at weeks 4 and 8 (P<0.05); HGF of groups C and D was significantly elevated at 4 and 8 weeks than groups A and B (P<0.05), in which the group D showed the highest level of HGF. According to tissue histologic observation, rat liver tissue structure of group A was clear and normal, tissue structure of group B was destroyed with obvious fibrous tissue hyperplasia and fatty change of hepatic cells; groups C and D showed a slighter liver tissue damage, cell necrosis and connective tissue hyperplasia in collect abbacy than group B with a trend of obvious improvement. Conclusions: Matrine can reduce TGF-beta 1 expression and enhance the activity of HGF, so as to realize the inhibition effect on liver fibrosis in rats.
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页码:390 / 393
页数:4
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