Quantitative detection of soybean in meat products by a TaqMan real-time PCR assay

被引：31
|
作者：
Soares, Sonia [1 ]
Amaral, Joana S. [1 ,2 ]
Oliveira, M. Beatriz P. P. [1 ]
Mafra, Isabel [1 ]
机构：
[1] Univ Porto, Fac Farm, Dept Ciencias Quim, P-4050313 Oporto, Portugal
[2] Inst Politecn Braganca, ESTiG, P-5301857 Braganca, Portugal
来源：
MEAT SCIENCE | 2014年 / 98卷 / 01期
关键词：
Soybean detection; Adulteration; Food allergen; Meat products; Real-time PCR; POLYMERASE-CHAIN-REACTION; SOY PROTEINS; MIXTURES; FOOD; DNA; QUANTIFICATION; AUTHENTICATION; ALLERGEN; PORK;
D O I：
10.1016/j.meatsci.2014.04.002
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
In the present work, we propose a normalised real-time quantitative PCR assay to determine the addition of soybean to meat products. The method proved to be a powerful tool for the quantification of soybean protein (dry basis) in the range of 0.01% to 6%, being successfully in-house validated. Its application was effective in the analysis of several meat products, indicating 2% of non-compliance with the food allergen labelling legislation, and some inconsistencies when comparing the declared with estimated amounts of soybean. This work highlights the importance of efficient tools to assess labelling statements of meat products, avoiding fraudulent practices. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：41 / 46
页数：6
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