G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila

被引：253

作者：

Evans, Cory J. ^{[1
]}

Olson, John M. ^{[1
]}

Ngo, Kathy T. ^{[1
]}

Kim, Eunha ^{[1
]}

Lee, Noemi E. ^{[1
]}

Kuoy, Edward ^{[1
]}

Patananan, Alexander N. ^{[1
]}

Sitz, Daniel ^{[1
]}

Tran, PhuongThao ^{[1
]}

Do, Minh-Tu ^{[1
]}

Yackle, Kevin ^{[1
]}

Cespedes, Albert ^{[1
]}

Hartenstein, Volker ^{[1
,2
,3
]}

Call, Gerald B. ^{[1
]}

Banerjee, Utpal ^{[1
,2
,3
,4
]}

机构：

[1] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90024 USA

[2] Univ Calif Los Angeles, Dept Biol Chem, Los Angeles, CA 90024 USA

[3] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90024 USA

[4] Univ Calif Los Angeles, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Los Angeles, CA USA

来源：

NATURE METHODS | 2009年 / 6卷 / 08期

基金：

美国国家卫生研究院;

关键词：

EXPRESSION; HEMATOPOIESIS; PROTEIN; EYE;

D O I：

10.1038/nmeth.1356

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.

引用

页码：603 / U70

页数：4

共 14 条

[1] New Drosophila transgenic reporters:: insulated P-element vectors expressing fast-maturing RFP [J].

Barolo, S ;

Castro, B ;

Posakony, JW .

BIOTECHNIQUES, 2004, 36 (03) :436-+

[2] Genomewide clonal. analysis of lethal mutations in the Drosophila melanogaster eye:: Comparison of the X chromosome and autosomes [J].

Call, Gerald B. ;

Olson, John M. ;

Chen, Jiong ;

Villarasa, Nikki ;

Ngo, Kathy T. ;

Yabroff, Allison M. ;

Cokus, Shawn ;

Pellegrini, Matteo ;

Bibikova, Elena ;

Bui, Chris ;

Cespedes, Albert ;

Chan, Cheryl ;

Chan, Stacy ;

Cheema, Amrita K. ;

Chhabra, Akanksha ;

Chitsazzadeh, Vida ;

Do, Minh-Tu ;

Fang, Q. Angela ;

Folick, Andrew ;

Goodstein, Gelsey L. ;

Huang, Cheng R. ;

Hung, Tony ;

Kim, Eunha ;

Kim, William ;

Kim, Yulee ;

Kohan, Emil ;

Kuoy, Edward ;

Kwak, Robert ;

Lee, Eric ;

Lee, JiEun ;

Lin, Henry ;

Liu, H-C. Angela ;

Moroz, Tatiana ;

Prasad, Tharani ;

Prashad, Sacha L. ;

Patananan, Alexander N. ;

Rangel, Alma ;

Rosselli, Desiree ;

Sidhu, Sohrab ;

Sitz, Daniel ;

Taber, Chelsea E. ;

Tan, Jingwen ;

Topp, Kasey ;

Tran, PhuongThao ;

Tran, Quynh-Minh ;

Unkovic, Mary ;

Wells, Maggie ;

Wickland, Jessica ;

Yackle, Kevin ;

Yavari, Amir .

GENETICS, 2007, 177 (02) :689-697

[3] Discovery-based science education:: Functional genomic dissection in Drosophila by undergraduate researchers [J].

Chen, J ;

Call, GB ;

Beyer, E ;

Bui, C ;

Cespedes, A ;

Chan, A ;

Chan, J ;

Chan, S ;

Chhabra, A ;

Dang, P ;

Deravanesian, A ;

Hermogeno, B ;

Jen, J ;

Kim, E ;

Lee, E ;

Lewis, G ;

Marshall, J ;

Regalia, K ;

Shadpour, F ;

Shemmassian, A ;

Spivey, K ;

Wells, M ;

Wu, J ;

Yamauchi, Y ;

Yavari, A ;

Abrams, A ;

Abramson, A ;

Amado, L ;

Anderson, J ;

Bashour, K ;

Bibikova, E ;

Bookatz, A ;

Brewer, S ;

Buu, N ;

Calvillo, S ;

Cao, J ;

Chang, AL ;

Chang, D ;

Chang, YL ;

Chen, YB ;

Choi, J ;

Chou, JL ;

Datta, S ;

Davarifar, A ;

Desai, P ;

Fabrikant, J ;

Farnad, S ;

Fu, K ;

Garcia, E ;

Garrone, N .

PLOS BIOLOGY, 2005, 3 (02) :207-209

[4]

Elliott David A., 2008, V420, P79, DOI 10.1007/978-1-59745-583-1_5

[5] Hand is a direct target of Tinman and GATA factors during Drosophila cardiogenesis and hematopoiesis [J].

Han, Z ;

Olson, EN .

DEVELOPMENT, 2005, 132 (15) :3525-3536

[6] GETDB, a database compiling expression patterns and molecular locations of a collection of Gal4 enhancer traps [J].

Hayashi, S ;

Ito, K ;

Sado, Y ;

Taniguchi, M ;

Akimoto, A ;

Takeuchi, H ;

Aigaki, T ;

Matsuzaki, F ;

Nakagoshi, H ;

Tanimura, T ;

Ueda, R ;

Uemura, T ;

Yoshihara, M ;

Goto, S .

GENESIS, 2002, 34 (1-2) :58-61

[7]

Ito K, 1997, DEVELOPMENT, V124, P761

[8] The Drosophila lymph gland as a developmental model of hematopoiesis [J].

Jung, SH ;

Evans, CJ ;

Uemura, C ;

Banerjee, U .

DEVELOPMENT, 2005, 132 (11) :2521-2533

[9] Hemese, a hemocyte-specific transmembrane protein, affects the cellular immune response in Drosophila [J].

Kurucz, E ;

Zettervall, CJ ;

Sinka, R ;

Vilmos, P ;

Pivarcsi, A ;

Ekengren, S ;

Hegedüs, Z ;

Ando, I ;

Hultmark, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) :2622-2627

[10] Tools for neuroanatomy and neurogenetics in Drosophila [J].

Pfeiffer, Barret D. ;

Jenett, Arnim ;

Hammonds, Ann S. ;

Ngo, Teri-T B. ;

Misra, Sima ;

Murphy, Christine ;

Scully, Audra ;

Carlson, Joseph W. ;

Wan, Kenneth H. ;

Laverty, Todd R. ;

Mungall, Chris ;

Svirskas, Rob ;

Kadonaga, James T. ;

Doe, Chris Q. ;

Eisen, Michael B. ;

Celniker, Susan E. ;

Rubin, Gerald M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (28) :9715-9720

← 1 2 →