Direct study of minor extra-virgin olive oil components without any sample modification. 1H NMR multisupression experiment: A powerful tool

被引：75

作者：

Ruiz-Aracama, Ainhoa ^{[1
]}

Goicoechea, Encarnacion ^{[1
]}

Guillen, Maria D. ^{[1
]}

机构：

[1] Univ Basque Country, UPV EHU, Lascaray Res Ctr, Food Technol,Fac Pharm, Paseo Univ 7, Vitoria 01006, Spain

来源：

FOOD CHEMISTRY | 2017年 / 228卷

关键词：

Extra-virgin olive oil (EVOO); Proton nuclear magnetic resonance (H-1 NMR); Multisuppression experiments; Aldehydic phenols; Sterols; Acyl groups; Squalene and terpenes; NUCLEAR-MAGNETIC-RESONANCE; PERFORMANCE LIQUID-CHROMATOGRAPHY; NMR-SPECTROSCOPY; SECOIRIDOID DERIVATIVES; LIGSTROSIDE AGLYCON; OLEA-EUROPAEA; IDENTIFICATION; SEPARATION; ESTERS; CONSTITUENTS;

D O I：

10.1016/j.foodchem.2017.02.009

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Proton Nuclear Magnetic Resonance (H-1 NMR) was employed to study monovarietal commercial Spanish extra-virgin olive oils (EVOO) (Arbequina, Arroniz, Cornicabra, Hojiblanca and Picual). Each sample was analyzed by a standard pulse and by an experiment suppressing the main lipid signals, enabling the detection of signals of minor components. The aim was to determine the possibilities of both H-1 NMR approaches to characterize EVOO composition, focusing on acyl groups, squalene, sterols, triterpene acids/esters, fatty alcohols, wax esters and phenols (lignans, tyrosol, hydroxytyrosol, oleocanthal, oleacein, oleokoronal, oleomissional, ligstrodials and oleuropeindials), and to determine hydrolysis and oxidation levels. The signal assignments (in deuterated chloroform) are thoroughly described, identifying for the first time those of the protons of esters of phytol and of geranylgeraniol. Correct signal assignment is fundamental for obtaining sound results when interpreting statistical data from metabolomic studies of EVOO composition and adulteration, making it possible to differentiate and classify oils. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：301 / 314

页数：14

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