Structure of the O-specific polysaccharide of Proteus mirabilis O11, another Proteus O-antigen containing an amide of D-galacturonic acid with L-threonine

被引：0

作者：

Arbatsky, NP

Shashkov, AS

Literacka, E

Widmalm, G

Kaca, W

Knirel, YA

机构：

[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 117913, Russia

[2] Univ Lodz, Inst Microbiol & Immunol, PL-90237 Lodz, Poland

[3] Polish Acad Sci, Ctr Microbiol & Virol, PL-92232 Lodz, Poland

[4] Univ Stockholm, Arrhenius Lab, Dept Organ Chem, S-10691 Stockholm, Sweden

来源：

CARBOHYDRATE RESEARCH | 2000年 / 323卷 / 1-4期

关键词：

Proteus mirabilis; D-galacturonoyl-L-threonine; O-antigen; O-specific polysaccharide; lipopolysaccharide; structure;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The O-specific polysaccharide of Proteus mirabilis O11 was studied by sugar analysis, Smith degradation, H-1 and C-13 NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, and H-1-detected H-1, C-13 HMQC experiments. The following structure of a pentasaccharide repeating unit of the polysaccharide was established: [GRAPHICS] where D-GalA6LThr is N-(D-galacturonoyl)-L-threonine. ELISA with anti-P. mirabilis O11 serum showed that D-GalA6LThr is of minor importance for manifesting the O11 immunospecificity. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：81 / 86

页数：6

共 23 条

[1] STUDY OF C-13H COUPLING-CONSTANTS IN HEXOPYRANOSES [J].