Toward a general chemical method for rapidly mapping multi-protein complexes

被引：22

作者：

Denison, C

Kodadek, T

机构：

[1] Univ Texas, SW Med Ctr, Ctr Biomed Intervent, Dallas, TX 75390 USA

[2] Univ Texas, SW Med Ctr, Dept Internal Med, Dallas, TX 75390 USA

[3] Univ Texas, SW Med Ctr, Dept Mol Biol, Dallas, TX 75390 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2004年 / 3卷 / 03期

关键词：

cross-linking; proteasome; PICUP;

D O I：

10.1021/pr034071j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ru(II)(bpy(2))(3)Cl-2+(2), ammonium persulfate, and visible light irradiation has been shown to rapidly and efficiently cross-link several interacting proteins. However, this methodology has not yet been used to map the architecture of large multi-protein complexes. In this study, this chemistry is applied to the crystallographically characterized yeast proteasome. The data obtained demonstrate both the method's increased generality and fidelity in comparison to traditional bifunctional cross-linking reagents, while also highlighting the future need for developing better analytical techniques to separate cross-linked products.

引用

收藏

页码：417 / 425

页数：9

相关论文

共 23 条

[1] The proteasome:: Paradigm of a self-compartmentalizing protease [J].

Baumeister, W ;

Walz, J ;

Zühl, F ;

Seemuller, E .

CELL, 1998, 92 (03) :367-380

[2] Protein cross-links: Universal isolation and characterization by isotopic derivatization and electrospray ionization mass spectrometry [J].

Chen, XH ;

Chen, YH ;

Anderson, VE .

ANALYTICAL BIOCHEMISTRY, 1999, 273 (02) :192-203

[3] Identical subunit topographies of human and yeast 20S proteasomes [J].

Dahlmann, B ;

Kopp, F ;

Kristensen, P ;

Hendil, KB .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1999, 363 (02) :296-300

[4] A protein-protein interaction map of the Caenorhabditis elegans 26S proteasome [J].

Davy, A ;

Bello, P ;

Thierry-Mieg, N ;

Vaglio, P ;

Hitti, J ;

Doucette-Stamm, L ;

Thierry-Mieg, D ;

Reboul, J ;

Boulton, S ;

Walhout, AJM ;

Coux, O ;

Vidal, M .

EMBO REPORTS, 2001, 2 (09) :821-828

[5] Bridging structural biology and genomics: assessing protein interaction data with known complexes [J].

Edwards, AM ;

Kus, B ;

Jansen, R ;

Greenbaum, D ;

Greenblatt, J ;

Gerstein, M .

TRENDS IN GENETICS, 2002, 18 (10) :529-536

[6] Chemistry for the analysis of protein-protein interactions: Rapid and efficient cross-linking triggered by long wavelength light [J].

Fancy, DA ;

Kodadek, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6020-6024

[7] The 19S regulatory particle of the proteasome is required for efficient transcription elongation by RNA polymerase II [J].

Ferdous, A ;

Gonzalez, F ;

Sun, LP ;

Kodadek, T ;

Johnston, SA .

MOLECULAR CELL, 2001, 7 (05) :981-991

[8] Functional organization of the yeast proteome by systematic analysis of protein complexes [J].

Gavin, AC ;

Bösche, M ;

Krause, R ;

Grandi, P ;

Marzioch, M ;

Bauer, A ;

Schultz, J ;

Rick, JM ;

Michon, AM ;

Cruciat, CM ;

Remor, M ;

Höfert, C ;

Schelder, M ;

Brajenovic, M ;

Ruffner, H ;

Merino, A ;

Klein, K ;

Hudak, M ;

Dickson, D ;

Rudi, T ;

Gnau, V ;

Bauch, A ;

Bastuck, S ;

Huhse, B ;

Leutwein, C ;

Heurtier, MA ;

Copley, RR ;

Edelmann, A ;

Querfurth, E ;

Rybin, V ;

Drewes, G ;

Raida, M ;

Bouwmeester, T ;

Bork, P ;

Seraphin, B ;

Kuster, B ;

Neubauer, G ;

Superti-Furga, G .

NATURE, 2002, 415 (6868) :141-147

[9] Structure of 20S proteasome from yeast at 2.4 angstrom resolution [J].

Groll, M ;

Ditzel, L ;

Lowe, J ;

Stock, D ;

Bochtler, M ;

Bartunik, HD ;

Huber, R .

NATURE, 1997, 386 (6624) :463-471

[10] Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology [J].

Gygi, SP ;

Corthals, GL ;

Zhang, Y ;

Rochon, Y ;

Aebersold, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (17) :9390-9395