High-throughput cloning of Campylobacter jejuni ORFs by in vivo recombination in Escherichia coli

被引:38
作者
Parrish, JR
Limjindaporn, T
Hines, JA
Liu, JY
Liu, GZ
Finley, RL
机构
[1] Wayne State Univ, Sch Med, Ctr Mol Med & Genet, Detroit, MI 48201 USA
[2] Wayne State Univ, Sch Med, Dept Biochem & Mol Biol, Detroit, MI 48201 USA
关键词
high-throughput cloning; Campylobacter; proteomics;
D O I
10.1021/pr0341134
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A rate-limiting and costly step in many proteomics analyses is the cloning of all of the ORFs for an organism into technique-specific vectors. Here, we describe the generation of a Campylobacter jejuni expression clone set using a high-throughput cloning approach based on recombination in E. coli. The approach uses native E coli recombination functions and requires no in vitro enzymatic steps or special strains. Our results indicate that this approach is an efficient and economical alternative for high-throughput cloning.
引用
收藏
页码:582 / 586
页数:5
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