Upregulation of GRP78 and GRP94 and Its Function in Chemotherapy Resistance to VP-16 in Human Lung Cancer Cell Line SK-MES-1

被引：37

作者：

Zhang, Lichuan ^{[2
]}

Wang, Siyan

Wangtao ^{[2
]}

Wang, Yanyan

Wang, Jiarui

Jiang, Li

Li, Sheng

Hu, Xiujuan ^{[3
]}

Wang, Qi ^{[1
]}

机构：

[1] Dalian Med Univ, Dept Resp Med, Affiliated Hosp 2, Dalian, Peoples R China

[2] Dalian Univ Technol, Dept Resp Med, Affiliated Zhongshan Hosp, Dalian, Peoples R China

[3] Thorax Hosp Tianjin, Dept Resp Med, Tianjin, Peoples R China

来源：

CANCER INVESTIGATION | 2009年 / 27卷 / 04期

关键词：

GRP78; GRP94; Human lung cancer cell line SK-MES-1; VP-16; Resistance; ENDOPLASMIC-RETICULUM STRESS; DEATH PROGRAM; PROTEIN; INDUCTION; GLUCOSE; GLYCOPROTEIN; SUPPRESSION; EXPRESSION; INHIBITORS; ETOPOSIDE;

D O I：

10.1080/07357900802527239

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The upregulation of GRP78 and GRP94 under the induction of A23187 and its function in drug resistance to etoposide (VP-16) was investigated in human lung cancer cell line SK-MES-1. The expression of GRP78 and GRP94 induced by A23187 at different concentrations was analyzed by RT-PCR and Western blotting. Cell survival to VP-16 was determined using a colony-formation assay. The expression of GRP78 and GRP94 in the cells was found to correspond well with the cell survival to VP-16. The results showed that upregulation of GRP78 and GRP94 can significantly confer the chemoresistance to VP-16 in human lung cancer cell line SK-MES-1.

引用

页码：453 / 458

页数：6

共 9 条

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