Cloning and Expression Analysis of Cathepsin D in the Olive Flounder Paralichthys olivaceus

被引：10

作者：

Park, Eun-Mi ^{[1
]}

Kim, Young-Ok ^{[1
]}

Nam, Bo-Hye ^{[1
]}

Kong, Hee Jeong ^{[1
]}

Kim, Woo-Jin ^{[1
]}

Lee, Sang-Jun ^{[1
]}

Kim, Kyung-Kil ^{[1
]}

机构：

[1] Natl Fisheries Res & Dev Inst, Biotechnol Res Inst, Pusan 619705, South Korea

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 08期

关键词：

Paralichthys olivaceus; cathepsin D; gene expression; RT-PCR; MOLECULAR-CLONING; ONCORHYNCHUS-MYKISS; ASPARTIC PROTEASE; RAINBOW-TROUT; APOPTOSIS; INTERLEUKIN-1-BETA; CANCER; CELLS; CYTOKINES; CDNA;

D O I：

10.1271/bbb.80822

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated a homolog of cathepsin D from the cDNA library of the olive flounder kidney. The olive flounder cathepsin D transcript consisted of 1,733 bp that encoded a polypeptide of 396 amino acids. The overall similarity between olive flounder cathepsin D and other cathepsin Ds was very high, with the highest amino acid sequence identity to barramundi perch (89%). RT-PCR revealed that cathepsin D was expressed in almost all tissues, with high expression in the liver, intestine, kidney, skin, and spleen. The accumulation of cathepsin D mRNA after bacterial infection, as determined by RT-PCR, was constitutive and increased greatly after bacterial infection.

引用

页码：1856 / 1859

页数：4

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