Since the mainstream methods of hardware trojan detection all need a reference IC which definitely have no Hardware Trojan Horse (HTH) implanted in and hardly be obtained, a novel hardware trojan detection method that doesn't need to rely on a reference IC based on low toggle-rate nodes is proposed in this paper. The gate-level netlist after layout of AES circuits was extracted to verify the detection method. The results show that the proposed method which can effectively detect HTH circuit in the original circuit and navigate to a specific circuit structure.