SOI-Nanowire Biosensor for the Detection of Glioma-Associated miRNAs in Plasma

被引：16

作者：

Malsagova, Kristina A. ^{[1
]}

Pleshakova, Tatyana O. ^{[1
]}

Galiullin, Rafael A. ^{[1
]}

Kozlov, Andrey F. ^{[1
]}

Romanova, Tatyana S. ^{[1
]}

Shumov, Ivan D. ^{[1
]}

Popov, Vladimir P. ^{[2
]}

Tikhonenko, Fedor V. ^{[2
]}

Glukhov, Alexander V. ^{[3
]}

Smirnov, Alexander Yu. ^{[4
]}

Gadzhieva, Olga A. ^{[5
]}

Bashiryan, Boris A. ^{[5
]}

Shimansky, Vadim N. ^{[5
]}

Archakov, Alexander I. ^{[1
]}

Ivanov, Yuri D. ^{[1
]}

机构：

[1] Inst Biomed Chem, Moscow 119121, Russia

[2] Russian Acad Sci, Siberian Branch, Rzhanov Inst Semicond Phys, Novosibirsk 630090, Russia

[3] JSC Novosibirsk Plant Semicond Devices OKB, Novosibirsk 630082, Russia

[4] Russian Union Industrialists & Entrepreneurs, Moscow 109240, Russia

[5] Minist Hlth Russian Federat, NN Burdenko Natl Med Res Ctr Neurosurg, Fed State Autonomous Inst, Moscow 125047, Russia

来源：

CHEMOSENSORS | 2020年 / 8卷 / 04期

关键词：

miRNA; silicon-on-insulator; nanowire biosensor; cancer; glioma; DNA oligonucleotide (oDNA); nanowire; GENETIC PATHWAYS; CANCER CELLS; GLIOBLASTOMA; MICRORNA; MIGRATION; THERAPY; PROLIFERATION; EXPRESSION; APOPTOSIS; FIELD;

D O I：

10.3390/chemosensors8040095

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein, we report the development of a highly sensitive nanotechnology-based system-silicon-on-insulator nanowire biosensor for the revelation of microRNAs (miRNAs), associated with the development of glioma in the human. In this system, a sensor chip, bearing an array of silicon nanowire structures, is employed. The sensor chip is fabricated using a top-down technology. In our experiments reported herein, we demonstrated the detection of DNA oligonucleotide (oDNA), which represents a synthetic analogue of microRNA-363 associated with the development of glioma. To provide biospecific detection of the target oligonucleotides, the surface of the nanowire structures is modified with oligonucleotide probes; the latter are complementary to the target ones. The concentration limit of the target oligonucleotide detection, attained using our nanowire biosensor, is at the level of DL similar to 10(-17) M. The revelation of the elevated level of glioma-associated miRNA in plasma is also demonstrated.

引用

页码：1 / 11

页数：11

共 36 条

[1] Status Quo-Standard-of-Care Medical and Radiation Therapy for Glioblastoma [J].