Structure of the VAP-peptide (BmACP-6.7) gene in the silkworm, Bombyx mori and a possible regulation of its expression by BmFTZ-F1

被引：11

作者：

Shiomi, K

Niimi, T

Imai, K

Yamashita, O ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[2] Mie Univ, Fac Bioresources, Tsu, Mie 5140008, Japan

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2000年 / 30卷 / 02期

基金：

日本学术振兴会;

关键词：

VAP-peptide (BmACP-6.7) gene; BmFTZ-F1; ecdysteroid; wing disc; metamorphosis; Bombyx mori;

D O I：

10.1016/S0965-1748(99)00105-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The VAP-peptide (BmACP-6.7) is a hydrophobic peptide localized in adult cuticle of the silkworm, Bombyx mori. We isolated and characterized the VAP-peptide gene as a useful marker gene to analyze molecular mechanisms of terminal differentiation processes in the adult. The gene is composed of two exons interrupted by one intron. The 5' upstream promoter region is shown to bear a nucleotide sequence similar to the cia-element that is recognized and bound by the Bombyx mori FTZ-F1 protein (BmFTZ-F1). Expression of the BmFTZ-F1 gene preceded expression of the VAP-peptide gene and injection of 20-hydroxyecdysone suppressed the expression of both genes. An in vitro binding assay indicated direct interaction of BmFTZ-F1 with the VAP-peptide gene promoter sequence. Therefore, BmFTZ-F1 is proposed to be a possible factor regulating the stage- specific expression of the VAP-peptide gene towards adult life. (C) 2000 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：119 / 125

页数：7

共 30 条

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