ANXA1 enhances tumor proliferation and migration by regulating epithelial-mesenchymal transition and IL-6/JAK2/STAT3 pathway in papillary thyroid carcinoma

被引:32
作者
Zhao, Xin [1 ]
Ma, Weiguo [2 ]
Li, Xinyu [1 ]
Li, Haijun [1 ]
Li, Jin [1 ]
Li, Hongle [3 ]
He, Fucheng [1 ]
机构
[1] Zhengzhou Univ, Dept Med Lab, Affiliated Hosp 1, Zhengzhou 450000, Henan, Peoples R China
[2] Zhengzhou Univ, Dept Med Lab, Affiliated Canc Hosp, Zhengzhou 450008, Henan, Peoples R China
[3] Zhengzhou Univ, Dept Mol Pathol, Affiliated Canc Hosp, Zhengzhou 450008, Henan, Peoples R China
关键词
ANXA1; papillary thyroid cancer; epithelial-mesenchymal transition; IL-6; STAT3; FINE-NEEDLE-ASPIRATION; ANNEXIN A1; PROTEOMIC ANALYSIS; CANCER CELL; EXPRESSION; ESOPHAGEAL; EMT;
D O I
10.7150/jca.52171
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Annexin A1 (ANXA1) was discovered to show various effects during tumor initiation and development in a tumor-specific manner. However, the function of ANXA1 in papillary thyroid carcinoma (PTC) has not been reported. Methods: Bioinformatic analyses, RT-PCR and immunohistochemistry were employed to determine the ANXA1 expression level in PTC. Both gain- and loss-of-function studies, including CCK-8, EdU assay, transwell experiment and wound-healing assay were used to investigate the role of ANXA1 in PTC progression. GSEA enrichment analysis was utilized to explore the potential mechanisms of ANXA1 mediated downstream signaling, and ELISA, RT-PCR and western blot were used to confirm the relevance. Results: ANXA1 expression was prominently upregulated in PTC tumor tissues. Ectopic expression of ANXA1 expedited PTC cell proliferation, migration and invasion, whereas ANXA1 knockdown exhibited the opposing trends. Mechanistic investigations showed that ANXA1 regulated epithelial-mesenchymal transition (EMT) and activated the IL-6/JAK2/STAT3 pathway to contribute to PTC malignant behaviors. In particular, loss of ANXA1 retarded tumor burden and suppressed lung metastasis in vivo. Conclusions: In conclusion, our findings identified ANXA1 as a pivotal oncogene during PTC carcinogenesis and ANXA1 might function as a promising therapeutic target and prognostic marker for PTC.
引用
收藏
页码:1295 / 1306
页数:12
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