Enzymatic properties of cytochrome P450 catalyzing 3′-hydroxylation of naringenin from the white-rot fungus Phanerochaete chrysosporium

被引：27

作者：

Kasai, Noriyuki ^{[1
]}

Ikushiro, Shin-ichi ^{[1
]}

Hirosue, Shinji ^{[2
]}

Arisawa, Akira ^{[2
]}

Ichinose, Hirofumi ^{[3
]}

Wariishi, Hiroyuki ^{[3
]}

Ohta, Miho ^{[4
]}

Sakaki, Toshiyuki ^{[1
]}

机构：

[1] Toyama Prefectural Univ, Fac Engn, Dept Biotechnol, Toyama 9390398, Japan

[2] Mercian Corp, Bioresource Labs, Iwata, Shizuoka, Japan

[3] Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Dept Forest & Forest Prod Sci, Fukuoka 8128581, Japan

[4] Soai Univ, Suminoe Ku, Osaka 5590033, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 387卷 / 01期

关键词：

Cytochrome P450; P; chrysosporium; Naringenin hydroxylation; Dioxin; DIETARY FLAVONOIDS; IN-VITRO; ESTROGENIC FLAVONOIDS; CYP1A SUBFAMILY; RAT; ERIODICTYOL; METABOLISM; ANTIOXIDANTS; OXIDATION; ENZYMES;

D O I：

10.1016/j.bbrc.2009.06.134

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned full-length cDNAs of more than 130 cytochrome P450s (P450s) derived from Phanerochaete chrysosporium, and successfully expressed 70 isoforms using a co-expression system of P. chrysosporium P450 and yeast NADPH-P450 reductase in Saccharomyces cerevisiae. Of these P450s, a microsomal P450 designated as PcCYP65a2 consists of 626 amino acid residues with a molecular mass of 68.3 kDa. Sequence alignment of PcCYP65a2 and human CYP1A2 revealed a unique structure of PcCYP65a2. Functional analysis of PcCYP65a2 Using the recombinant S. cerevisiae cells demonstrated that this 11450 catalyzes 3'-hydroxylation of naringenin to yield eriodictyol, which has various biological and pharmacological properties. In addition, the recombinant S. cerevisiae cells expressing PcCYP65a2 metabolized such polyaromatic compounds as dibenzo-p-dioxin (DD), 2-monochloroDD, biphenyl, and naphthalene. These results Suggest that PcCYP65a2 is practically Useful For both bioconversion and bioremediation. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：103 / 108

页数：6

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