TLP1: A gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family

被引:366
作者
Nakayama, JI [1 ]
Saito, M [1 ]
Nakamura, H [1 ]
Matsuura, A [1 ]
Ishikawa, F [1 ]
机构
[1] MITSUBISHI CHEM CORP,YOKOHAMA RES CTR,PHARMACEUT LAB 2,AOBA KU,YOKOHAMA,KANAGAWA 227,JAPAN
关键词
D O I
10.1016/S0092-8674(00)81933-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240 and p230 were copurified with telomerase activity in a series of extensive purification experiments. These results strongly suggest that the TLP1 proteins are components of, or are closely associated with, the rat telomerase. A pulse-chase experiment showed that p240 is modified to p230 in vivo. p230 was the dominant form in telomerase-positive cells, suggesting that modification of the TLP1 protein may regulate telomerase activity in vivo.
引用
收藏
页码:875 / 884
页数:10
相关论文
共 47 条
[11]   CHROMATIN STRUCTURE OF THE MOLECULAR ENDS OF OXYTRICHA MACRONUCLEAR DNA - PHASED NUCLEOSOMES AND A TELOMERIC COMPLEX [J].
GOTTSCHLING, DE ;
CECH, TR .
CELL, 1984, 38 (02) :501-510
[12]   A TELOMERIC SEQUENCE IN THE RNA OF TETRAHYMENA TELOMERASE REQUIRED FOR TELOMERE REPEAT SYNTHESIS [J].
GREIDER, CW ;
BLACKBURN, EH .
NATURE, 1989, 337 (6205) :331-337
[13]   THE TELOMERE TERMINAL TRANSFERASE OF TETRAHYMENA IS A RIBONUCLEOPROTEIN ENZYME WITH 2 KINDS OF PRIMER SPECIFICITY [J].
GREIDER, CW ;
BLACKBURN, EH .
CELL, 1987, 51 (06) :887-898
[14]   Telomere length regulation [J].
Greider, CW .
ANNUAL REVIEW OF BIOCHEMISTRY, 1996, 65 :337-365
[15]   IDENTIFICATION OF A SPECIFIC TELOMERE TERMINAL TRANSFERASE-ACTIVITY IN TETRAHYMENA EXTRACTS [J].
GREIDER, CW ;
BLACKBURN, EH .
CELL, 1985, 43 (02) :405-413
[16]   TELOMERES SHORTEN DURING AGING OF HUMAN FIBROBLASTS [J].
HARLEY, CB ;
FUTCHER, AB ;
GREIDER, CW .
NATURE, 1990, 345 (6274) :458-460
[17]   TELOMERES AND TELOMERASE IN AGING AND CANCER [J].
HARLEY, CB ;
VILLEPONTEAU, B .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 1995, 5 (02) :249-255
[18]   TELOMERE REDUCTION IN HUMAN COLORECTAL-CARCINOMA AND WITH AGING [J].
HASTIE, ND ;
DEMPSTER, M ;
DUNLOP, MG ;
THOMPSON, AM ;
GREEN, DK ;
ALLSHIRE, RC .
NATURE, 1990, 346 (6287) :866-868
[19]  
HO PLC, 1994, SCIENCE, V266, P2011
[20]   Control of telomere growth by interactions of RAP1 with the most distal telomeric repeats [J].
Krauskopf, A ;
Blackburn, EH .
NATURE, 1996, 383 (6598) :354-357