TLP1: A gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family

被引：366

作者：

Nakayama, JI ^{[1
]}

Saito, M ^{[1
]}

Nakamura, H ^{[1
]}

Matsuura, A ^{[1
]}

Ishikawa, F ^{[1
]}

机构：

[1] MITSUBISHI CHEM CORP,YOKOHAMA RES CTR,PHARMACEUT LAB 2,AOBA KU,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

CELL | 1997年 / 88卷 / 06期

关键词：

D O I：

10.1016/S0092-8674(00)81933-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240 and p230 were copurified with telomerase activity in a series of extensive purification experiments. These results strongly suggest that the TLP1 proteins are components of, or are closely associated with, the rat telomerase. A pulse-chase experiment showed that p240 is modified to p230 in vivo. p230 was the dominant form in telomerase-positive cells, suggesting that modification of the TLP1 protein may regulate telomerase activity in vivo.

引用

页码：875 / 884

页数：10

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