Cytokine mRNA expression in B cells from bovine leukemia virus-infected cattle with persistent lymphocytosis

被引：20
作者：
Amills, M [1 ]
Norimine, J [1 ]
Olmstead, CA [1 ]
Lewin, HA [1 ]
机构：
[1] Univ Illinois, Dept Anim Sci, Immunogenet Lab, Edward R Madigan Lab, Urbana, IL 61801 USA
来源：
CYTOKINE | 2004年 / 28卷 / 01期
关键词：
bovine leukemia virus; cytokine; B cell; persistent lymphocytosis;
D O I：
10.1016/j.cyto.2004.06.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have characterized the expression of six cytokine mRNAs in highly purified B cells from bovine leukemia virus (BLV)-infected cows with persistent lymphocytosis. Selected cytokine mRNAs included those encoding tumor necrosis factor (TNF), lymphotoxin-alpha (LT-alpha), transforming growth factor-beta(1) (TGF-beta(1)), interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and interleukin-10 (IL-10). Fresh B cells from cows with persistent lymphocytosis constitutively transcribed TNF, LT-alpha and TGF-beta, mRNAs. Although IL-1beta, IL-6 and IL-10 mRNAs were barely detectable in fresh B cells from cows with persistent lymphocytosis, transcripts encoding these cytokines were strongly and rapidly upregulated in B cells after cell culture. Results from this study provide the first evidence that B cells infected with BLV express specific cytokine mRNAs in vivo. (C) 2004 Elsevier Ltd. All rights reserved.
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收藏
页码：25 / 28
页数：4
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