A surface plasmon resonance-based solution affinity assay for heparan sulfate-binding proteins

被引：48

作者：

Cochran, Siska ^{[1
]}

Li, Cai Ping ^{[1
]}

Ferro, Vito ^{[1
]}

机构：

[1] Progen Pharmaceut Ltd, Drug Design Grp, Darra, Qld, Australia

来源：

GLYCOCONJUGATE JOURNAL | 2009年 / 26卷 / 05期

关键词：

Heparan sulfate-binding proteins; heparin; solution affinity assay; surface plasmon resonance; FIBROBLAST-GROWTH-FACTOR; GLYCOSAMINOGLYCAN OLIGOSACCHARIDES; SUCROSE OCTASULFATE; BIOLOGICAL-ACTIVITY; CELL-PROLIFERATION; LIGAND-BINDING; MODEL; ANGIOGENESIS; INHIBITORS; DOMAIN;

D O I：

10.1007/s10719-008-9210-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A surface plasmon resonance-based solution affinity assay is described for measuring the K (d) of binding of heparin/heparan sulfate-binding proteins with a variety of ligands. The assay involves the passage of a pre-equilibrated solution of protein and ligand over a sensor chip onto which heparin has been immobilised. Heparin sensor chips prepared by four different methods, including biotin-streptavidin affinity capture and direct covalent attachment to the chip surface, were successfully used in the assay and gave similar K (d) values. The assay is applicable to a wide variety of heparin/HS-binding proteins of diverse structure and function (e.g., FGF-1, FGF-2, VEGF, IL-8, MCP-2, ATIII, PF4) and to ligands of varying molecular weight and degree of sulfation (e.g., heparin, PI-88, sucrose octasulfate, naphthalene trisulfonate) and is thus well suited for the rapid screening of ligands in drug discovery applications.

引用

页码：577 / 587

页数：11

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