MALDI-TOF mass spectrometry: Evaluation of the preanalytical phase for identification of molds

被引：3
|
作者：
Maldonado, Ivana [1 ]
Garcia Ramirez, Dolores [1 ]
Striebeck, Pablo [1 ]
Lafage, Marcelo [1 ]
Fernandez Canigia, Liliana [1 ]
机构：
[1] Hosp Aleman, Lab Microbiol, Buenos Aires, DF, Argentina
来源：
REVISTA ARGENTINA DE MICROBIOLOGIA | 2017年 / 49卷 / 01期
关键词：
Molds; Mass spectrometry; Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; DESORPTION IONIZATION-TIME; SPECIES IDENTIFICATION; FUNGI; ASPERGILLUS; MYCOLOGY;
D O I：
10.1016/j.ram.2016.10.001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In order to optimize the identification of molds with MALDI-TOF MS, three protein extraction-methodologies were evaluated against 44 isolates: water extraction ( WE), zirconium extraction (ZE) and the provider's recommended method (PRM). Two data bases were compared, Bruker (BK) and Bruker + National Institutes of Health. Considering both databases, results were respectively as follows: correct identification (CI) at gender level, 10 and 16 by WE; 27 and 32 by ZE and 18 and 23 by PRM; CI at species level, 5 and 7 by WE; 17 and 20 by ZE Con and 11 and 14 by PRM; non-reliable identification, 18 and 12 by WE; 9 and 4 by ZE andby PRM. No peaks were observed in 16 by WE, 8 by ZE and 17 by PRM. ZE showed the bestperfomance (p < 0.05). (C) 2016 Asociaci ' on Argentina de Microbiologia. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY-NC-ND license
引用
收藏
页码：7 / 14
页数：8
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