The rice gene OsZFP6 functions in multiple stress tolerance responses in yeast and Arabidopsis

被引：15

作者：

Guan, Qing-jie ^{[1
,3
]}

Wang, Li-feng ^{[2
]}

Bu, Qing-yun ^{[1
]}

Wang, Zhen-yu ^{[1
]}

机构：

[1] Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Harbin 150081, Heilongjiang, Peoples R China

[2] CATAS, Rubber Res Inst, State Key Lab Incubat Base Cultivat & Physiol Tro, Key Lab Biol & Genet Resources Rubber Tree,Minist, Danzhou 571737, Hainan, Peoples R China

[3] Northeast Forestry Univ, ASNESC, Harbin 150040, Heilongjiang, Peoples R China

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2014年 / 82卷

基金：

中国博士后科学基金;

关键词：

Zinc finger protein; Abiotic stress; Rice; Alkali; H2O2; ZINC-FINGER PROTEIN; TRANSCRIPTION FACTORS; ORYZA-SATIVA; VARIANTS; ENCODES; SUBSET; GROWTH; DOMAIN; COLD; ACID;

D O I：

10.1016/j.plaphy.2014.04.021

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The role of zinc finger proteins in organismal stress conditions has been widely reported. However, little is known concerning the function of CCHC-type zinc finger proteins in rice. In this study, OsZFP6, a rice CCHC-type zinc finger protein 6 gene, was cloned from rice using RT-PCR. The OsZFP6 protein contains 305 amino acids and a conserved zinc finger domain and is localised to the nucleus. Southern blot analysis revealed that a single copy was encoded in the rice genome. OsZFP6 expression was increased by abiotic stress, including salt (NaCl), alkali (NaHCO3) and H2O2 treatment. When OsZFP6 was transformed into yeast, the transgenic yeast showed significantly increased resistance to NaHCO3 compared to the control. Moreover, Arabidopsis transgenic plants overexpressing OsZFP6 were more tolerant to both NaHCO3 and H2O2 treatments. Overall, we uncovered a role for OsZFP6 in abiotic stress responses and identified OsZFP6 as a putatively useful gene for developing crops with increased alkali and H2O2 tolerance. (C) 2014 Elsevier Masson SAS. All rights reserved.

引用

页码：1 / 8

页数：8

共 40 条

[1] A TRYPANOSOMAL CCHC-TYPE ZINC-FINGER PROTEIN WHICH BINDS THE CONSERVED UNIVERSAL SEQUENCE OF KINETOPLAST DNA MINICIRCLES - ISOLATION AND ANALYSIS OF THE COMPLETE CDNA FROM CRITHIDIA-FASCICULATA [J].