Screening for topoisomerase I binding compounds by high-performance liquid chromatography-mass spectrometry

被引：13

作者：

Zhang, H

Gu, Q

Liang, XL

Pan, YJ ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Chem, Hangzhou, Peoples R China

[2] Hangzhou Univ Commerce, Dept Food Sci, Hangzhou, Peoples R China

[3] Zhejiang Univ, Dept Biol, Hangzhou, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2004年 / 329卷 / 02期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/j.ab.2004.03.057

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new rapid compound screening approach for topoisomerase I binding activity is presented. DNA topoisomerase I is used as a target protein to capture binding compounds from a mixture of combinatorial compounds by bioaffinity ultrafiltration. Using high-performance liquid chromatography combined with electrospray ionization mass spectrometry, small-molecule active compounds were identified. We also have successfully applied this method to identifying compounds from cells grown in culture. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：173 / 179

页数：7

共 14 条

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Randall W.N., 1995, ANAL CHEM, V67, P1153

[12] ISOLATION AND IDENTIFICATION OF CAMPTOTHECIN FROM CELLS OF CAMPTOTHECA-ACUMINATA SUSPENSION CULTURES [J].