Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

被引：49

作者：

Griffiths, Rian L. ^{[1
]}

Dexter, Alex ^{[1
]}

Creese, Andrew J. ^{[1
]}

Cooper, Helen J. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

来源：

ANALYST | 2015年 / 14卷 / 20期

基金：

英国工程与自然科学研究理事会;

关键词：

TOP-DOWN; INTACT PROTEINS; METABOLISM; PROTEOMICS; SAMPLES; PLATFORM; TISSUE; LIPIDS; TOOL; MS;

D O I：

10.1039/c5an00933b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Liquid extraction surface analysis (LESA) is a surface sampling technique that allows electrospray mass spectrometry analysis of a wide range of analytes directly from biological substrates. Here, we present LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots on filter paper. Incorporation of FAIMS in the workflow enables gas-phase separation of lipid and protein molecular classes, enabling analysis of both haemoglobin and a range of lipids (phosphatidylcholine or phosphatidylethanolamine, and sphingomyelin species) from a single extraction sample. The work has implications for multiplexed clinical assays of multiple analytes.

引用

页码：6879 / 6885

页数：7

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