Brain-derived neurotrophic factor in the amygdala mediates susceptibility to fear conditioning

被引:60
作者
Chou, Dylan [1 ,2 ]
Huang, Chiung-Chun [1 ]
Hsu, Kuei-Sen [1 ,2 ]
机构
[1] Natl Cheng Kung Univ, Coll Med, Dept Pharmacol, Tainan 701, Taiwan
[2] Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci, Tainan 701, Taiwan
关键词
Fear conditioning; Social defeat stress; BDNF; Anxiety; Amygdala; INDIVIDUAL-DIFFERENCES; MOLECULAR-MECHANISMS; SOCIAL DEFEAT; DELTA-FOSB; STRESS; MICE; MEMORY; RESILIENCE; RESPONSES; PROTEIN;
D O I
10.1016/j.expneurol.2014.02.016
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Fear conditioning in animals has been used extensively to model clinical anxiety disorders. While individual animals exhibit marked differences in their propensity to undergo fear conditioning, the physiologically relevant mediators have not yet been fully characterized. Here, we demonstrate that C57BL/6 inbred mouse strain subjected to a regimen of chronic social defeat stress (CSDS) can be separated into susceptible and resistant subpopulations that display different levels of fear responses in an auditory fear conditioning paradigm. Susceptible mice had significantly more c-Fos protein expression in neurons of the basolateral amygdala (BIA) following CSDS and showed exaggerated conditioned fear responses, while there were no significant differences between groups in innate anxiety- and depressive-like behaviors. Through the use of conditional brain-derived neurotrophic factor (BDNF) knockout strategies, we find that elevated BLA BDNF level following fear conditioning training is a key mediator contributing to determine the levels of conditioned fear responses. Our results also show that relative to susceptible mice, resistant mice had a much faster recovery from conditioned stimuli-induced cardiovascular and corticosterone responses. Systemic administration of norepinephrine reuptake inhibitor atomoxetine increased c-Fos protein expression in BLA neurons following fear conditioning training and promoted the expression of conditioned fear in resistant mice. Conversely, administration of beta-adrenergic receptor antagonist propranolol reduced fear conditioning training-induced c-Fos protein expression in BIA neurons and reduced conditioned fear responses in susceptible mice. These findings reveal a novel role for the BDNF signaling within the BLA in mediating individual differences in autonomic, neuroendocrine and behavioral reactivity to fear conditioning. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:19 / 29
页数:11
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