Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

被引：8

作者：

Ren, Xueli ^{[1
,2
]}

Akimoto, Jun ^{[1
]}

Miyatake, Hideyuki ^{[1
]}

Tada, Seiichi ^{[3
]}

Zhu, Liping ^{[1
]}

Mao, Hongli ^{[1
]}

Isoshima, Takashi ^{[1
]}

Mueller, Stefan ^{[3
]}

Kim, Seong Min ^{[1
]}

Zhou, Yulai ^{[2
]}

Ito, Yoshihiro ^{[1
,3
]}

机构：

[1] RIKEN, Cluster Pioneering Res, Nano Med Engn Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan

[2] Jilin Univ, Sch Pharmaceut Sci, 1266 Fujin Rd, Changchun 130021, Jilin, Peoples R China

[3] RIKEN, Ctr Emergent Matter Sci, Emergent Bioengn Mat Res Team, Wako, Saitama 3510198, Japan

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2019年 / 7卷 / 27期

基金：

日本学术振兴会;

关键词：

IN-VITRO; FIBRIN MATRICES; PROLIFERATION; STRATEGIES; AFFINITY; RELEASE; DESIGN; INTERNALIZATION; IMMOBILIZATION; SURFACES;

D O I：

10.1039/c9tb00407f

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Vascular endothelial growth factors (VEGFs) play an important role in tissue repair and regeneration. In this study, VEGFs were immobilised by photo-reactive gelatine by photo-irradiation and the effect of immobilisation was quantitatively investigated. Immobilised VEGFs enhanced endothelial cell migration and growth more than soluble VEGFs. In addition, the activation effects depended on the VEGF isoforms. Prolonged activation of phosphorylation sites in the VEGF receptor, which is associated with migration and proliferation, was observed on the immobilised VEGF surfaces. The results indicated that photo-immobilised VEGFs induced high cell activation by stimulating phosphorylation of the VEGF receptor, VEGFR2, which activated multiple cell functions.

引用

页码：4272 / 4279

页数：8

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