Fast Determination of Antioxidant Capacity of Food Samples Using Continuous Amperometric Detection on Polyester Screen-printed Graphitic Electrodes

被引：6

作者：

Arantes, Iana V. S. ^{[1
]}

Stefano, Jessica S. ^{[1
]}

Sousa, Raquel M. F. ^{[1
]}

Richter, Eduardo M. ^{[1
]}

Foster, Christopher W. ^{[2
]}

Banks, Craig E. ^{[2
]}

Munoz, Rodrigo A. A. ^{[1
]}

机构：

[1] Univ Fed Uberlandia, Ave Joao Naves de Avila 2121, BR-38408100 Uberlandia, MG, Brazil

[2] Manchester Metropolitan Univ, Fac Sci & Engn, Manchester M1 5GD, Lancs, England

来源：

ELECTROANALYSIS | 2018年 / 30卷 / 06期

关键词：

Amperometry; DPPH; Edible oil; Portable method; Screen-printed electrode; Tocopherol; BATCH-INJECTION-ANALYSIS; PORTABLE ELECTROCHEMICAL SYSTEM; PHARMACEUTICAL FORMULATIONS; VOLTAMMETRIC BEHAVIOR; FLOW-INJECTION; OLIVE OILS; IN-VITRO; DPPH; LIQUID; ASSAYS;

D O I：

10.1002/elan.201800122

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The determination of antioxidant capacity of food samples using disposable polyester screen-printed graphitic macroelectrodes (SPGE) coupled with a batch-injection cell based on the measurement of the consumption of 2,2-diphenyl-1-picrylhydrazyl (DPPH.) is reported. The SPGEs are organic-resistant electrodes and thus compatible with food samples and organic solvents used to dissolve DPPH.. A micropipette controlled the release (193Ls(-1)) of sample (150L) upon the sensor/working electrode immersed in electrolyte (1:1 ethanol/methanol containing 0.05molL(-1) LiCl). The first demonstration of this protocol was devoted to the analysis of edible oils. The remaining DPPH. was amperometrically detected at +0.1V (vs. pseudo AgCl). The antioxidant capacity (percentage of DPPH. scavenging and mg equivalent of tocopherol) was obtained using the proposed and spectrophotometric methods. The results were in agreement according to t-test (confidence level of 95%). The method is precise (RSD=2.3%, n=12), fast (180h(-1)), presents low detection limit of DPPH. (1molL(-1)), and allows on-site analysis. Moreover, the proposed method can be extended to the analysis of antioxidant capacity of other samples of agricultural and food interests.

引用

页码：1192 / 1197

页数：6