Probing Cellular Molecules with PolyA-Based Engineered Aptamer Nanobeacon

被引：102

作者：

Chen, Lizhen ^{[1
]}

Chao, Jie ^{[2
]}

Qu, Xiangmeng ^{[1
]}

Zhang, Hongbo ^{[3
]}

Zhu, Dan ^{[2
]}

Su, Shao ^{[2
]}

Aldalbahi, Ali ^{[4
]}

Wang, Lianhui ^{[2
]}

Pei, Hao ^{[1
]}

机构：

[1] East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Green Chem & Chem Proc, 500 Dongchuan Rd, Shanghai 200241, Peoples R China

[2] Nanjing Univ Posts & Telecommun, Inst Adv Mat, Nanjing 210023, Jiangsu, Peoples R China

[3] Univ Helsinki, Fac Pharm, Div Pharmaceut Chem & Technol, FI-00014 Helsinki, Finland

[4] King Saud Univ, Dept Chem, Riyadh 11451, Saudi Arabia

来源：

ACS APPLIED MATERIALS & INTERFACES | 2017年 / 9卷 / 09期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

nanobeacon; aptamer; spherical nucleic acid; cellular analysis; surface engineering self-assembly; BACKGROUND SIGNAL PLATFORM; GOLD NANOPARTICLES; COLORIMETRIC DETECTION; DNA; ADENOSINE; ATP; NANOPROBES; OLIGONUCLEOTIDES; BIOSENSOR; BEACON;

D O I：

10.1021/acsami.6b16764

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Adenosine triphosphate (ATP) is a central metabolite that is of critical importance in many cellular processes. The development of sensitive and selective methods for the detection of ATP level in vivo is crucial in diagnostic and theranostic applications. In this work, we have developed a polyA-based aptamer nanobeacon (PAaptNB) with improved efficiency and speed of ATP analysis. We found that the dissociation constants and competitive binding kinetics of the PAaptNB could be programmably regulated by adjusting the polyA length. When the polyA length reached to 30 bases, a 10 mu M detection limit for ATP assay with PAaptNB can be achieved (similar to 10-fold improvement compared with the conventional thiol-based aptamer nanobeacon). The feasibility of the PAaptNB for in vivo assay was further demonstrated by imaging intracellular ATP molecules. This study provides a new strategy to construct high-efficiency and high-speed biosensors for cellular molecules analysis, which holds great potential in bioanalysis and theranostic applications.

引用

页码：8014 / 8020

页数：7

共 51 条

[21] Structure-switching signaling aptamers [J].