Activity and Resistance Assessment of a New OSBP Inhibitor, R034-1, in Phytophthora capsici and the Detection of Point Mutations in PcORP1 that Confer Resistance

被引：24

作者：

Lin, Dong ^{[1
]}

Xue, Zhaolin ^{[1
]}

Miao, Jianqiang ^{[2
]}

Huang, Zhongqiao ^{[1
]}

Liu, Xili ^{[1
,2
]}

机构：

[1] China Agr Univ, Beijing 100193, Peoples R China

[2] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712110, Shaanxi, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2020年 / 68卷 / 47期

基金：

中国国家自然科学基金;

关键词：

Phytophthora capsici; R034-1; high activity; resistance assessment; point mutation; UNDERSTANDING MOLECULAR-MECHANISMS; MEFENOXAM INSENSITIVITY; FUNGICIDE RESISTANCE; VEGETABLE CROPS; SDHI FUNGICIDES; OXATHIAPIPROLIN; GENE; SENSITIVITY; PEPPER; MANAGEMENT;

D O I：

10.1021/acs.jafc.0c05531

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

R034-1 is a new member of the piperidinyl thiazole isoxazoline class of fungicides that shows high activity against most plant-pathogenic oomycetes and could effectively inhibit several developmental stages of Phytophthora capsici. Here, the potential resistance risk for R034-1 was evaluated in P. capsici. The baseline sensitivities of 135 isolates to R034-1 showed a unimodal curve, with a mean EC50 value of 0.004 mu g/mL. Twelve resistant mutants were generated by fungicide adaptation and displayed lower fitness compared to parental isolates, which suggests that the resistance risk of P. capsici to R034-1 is low. R034-1 and oxathiapiprolin are structurally related, and resistant isolates display cross-resistance to both compounds, suggesting that these fungicides may target the same oxysterol binding protein. Comparison of PcORP1 genes in the resistant mutants and their parental isolates revealed (N767S, N767I, and G700V) amino acid substitutions in the R034-1 resistant mutant. Causality was functionally validated using site-directed mutagenesis of the target gene using the CRISPR/Cas9 system.

引用

页码：13651 / 13660

页数：10

共 45 条

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