Determination of acid phosphatase activity in a double injection flow system with electrochemical detection

被引：8
|
作者：
Castanón-Fernández, J [1 ]
Fernández-Abedul, MT [1 ]
Costa-García, A [1 ]
机构：
[1] Univ Oviedo, Dept Quim Fis & Analit, Asturias, Spain
来源：
ANALYTICA CHIMICA ACTA | 2000年 / 406卷 / 02期
关键词：
acid phosphatase; double-injection; stopped-flow; FIA-ECD;
D O I：
10.1016/S0003-2670(99)00772-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An enzyme assay for the measurement of total and prostatic acid phosphatase activity by flow injection analysis with electrochemical detection has been developed. The substrate (alpha-naphthyl phosphate) and enzyme are simultaneously injected to the system by means of a double injection valve. The alpha-naphtol produced after stopping the flow is amperometrically quantified. The calibration curve is linear from 0.01 to 1.3 u/l with a limit of detection of 0.006 u/l. Results were compared with that obtained spectrophotometrically. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：225 / 232
页数：8
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