Salmonella pathogenicity island 2-dependent evasion of the phagocyte NADPH oxidase

被引：432

作者：

Vazquez-Torres, A

Xu, YS

Jones-Carson, J

Holden, DW

Lucia, SM

Dinauer, MC

Mastroeni, P

Fang, FC ^{[1
]}

机构：

[1] Univ Colorado, Hlth Sci Ctr, Dept Med, Denver, CO 80262 USA

[2] Univ Colorado, Hlth Sci Ctr, Dept Pathol, Denver, CO 80262 USA

[3] Univ Colorado, Hlth Sci Ctr, Dept Microbiol, Denver, CO 80262 USA

[4] Univ London Imperial Coll Sci Technol & Med, Sch Med, Dept Infect Dis, London W12 0NN, England

[5] Indiana Univ, Sch Med, Indianapolis, IN 46202 USA

[6] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2BZ, England

来源：

SCIENCE | 2000年 / 287卷 / 5458期

关键词：

D O I：

10.1126/science.287.5458.1655

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A type III protein secretion system encoded by Salmonella pathogenicity island 2 (SPI2) has been found to be required for virulence and survival within macrophages. Here, SPI2 was shown to allow Salmonella typhimurium to avoid NADPH oxidase-dependent killing by macrophages. The ability of SPI2-mutant bacteria to survive in macrophages and to cause Lethal infection in mice was restored by abrogation of the NADPH oxidase-dependent respiratory burst. Ultrastructural and immunofluorescence microscopy demonstrated efficient Localization of the NADPH oxidase in the proximity of vacuoles containing SPI2-mutant but not wild-type bacteria, suggesting that SPI2 interferes with trafficking of oxidase-containing vesicles to the phagosome.

引用

页码：1655 / 1658

页数：4

共 45 条

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