Phenotypic traits of genetically closely related Leuconostoc spp.

被引:3
作者
Pogacic, Tomislav [1 ,2 ,3 ]
Chuat, Victoria [1 ,2 ]
Madec, Marie-Noelle [1 ,2 ]
Samarzija, Dubravka [3 ]
Lortal, Sylvie [1 ,2 ]
Valence, Florence [1 ,2 ]
机构
[1] INRA, CIRM BIA, UMR1253, F-35000 Rennes, France
[2] AGROCAMPUS OUEST, UMR1253, F-35000 Rennes, France
[3] Univ Zagreb, Fac Agr, Dept Dairy Sci, Zagreb 10000, Croatia
关键词
LACTIC-ACID BACTERIA; GENUS LEUCONOSTOC; DAIRY LEUCONOSTOC; GENOME SEQUENCE; PSEUDOMESENTEROIDES; IDENTIFICATION; STRAINS; MESENTEROIDES; DIVERSITY; CHEESES;
D O I
10.1016/j.idairyj.2014.05.014
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The aim of the study was to evaluate intercluster and intracluster phenotypic and genetic diversity of Leuconostoc spp. PFGE and RAPD-PCR were used to evaluate molecular diversity. Carbohydrate metabolism of the isolates was evaluated using API 50CH. The study demonstrated that genetically closely related isolates will not necessarily demonstrate the same phenotypic relatedness, indicating that phenotypic traits are isolate specific. Therefore, genetic selection of isolates by clustering for further phenotypic characterisation of representatives, might lead to loss of important phenotypic diversity. Furthermore, PFGE was shown to be an efficient molecular tool for the differentiation of Leuconostoc mesenteroides subsp. cremoris and Leuconostoc pseudomesenteroides, that has not been reported previously. Additionally, amygdaline, arbutine, L-arabinose and mannitol can be important fermentation traits for phenotypic differentiation of Ln. mesenteroides subsp. cremoris and Ln. pseudomesenteroides. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:96 / 101
页数:6
相关论文
共 32 条
[1]   Thermal resistance of bacteriophages attacking flavour-producing dairy Leuconostoc starter cultures [J].
Atamer, Zeynep ;
Ali, Yahya ;
Neve, Horst ;
Heller, Knut J. ;
Hinrichs, Joerg .
INTERNATIONAL DAIRY JOURNAL, 2011, 21 (05) :327-334
[2]  
Björkroth KJ, 1998, APPL ENVIRON MICROB, V64, P3313
[3]   Characterization of glucan-producing Leuconostoc strains isolated from sourdough [J].
Bounaix, Marie-Sophie ;
Gabriel, Valerie ;
Robert, Herve ;
Morel, Sandrine ;
Rernaud-Simeon, Magali ;
Gabriel, Bruno ;
Fontagne-Faucher, Catherine .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2010, 144 (01) :1-9
[4]   Molecular characterization and aromatic potential of Debaryomyces hansenii strains isolated from naturally fermented sausages [J].
Cano-Garcia, Liliana ;
Flores, Monica ;
Belloch, Carmela .
FOOD RESEARCH INTERNATIONAL, 2013, 52 (01) :42-49
[5]   Leuconostoc pseudomesenteroides as a cause of nosocomial urinary tract infections [J].
Cappelli, EA ;
Barros, RR ;
Camello, TCF ;
Teixeira, LM ;
Merquior, VLC .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (12) :4124-4126
[6]   Adventitious dairy Leuconostoc strains with interesting technological and biological properties useful for adjunct starters [J].
Cardamone, Luisina ;
Quiberoni, Andrea ;
Javier Mercanti, Diego ;
Fornasari, Maria Emanuela ;
Alberto Reinheimer, Jorge ;
Marta Guglielmotti, Daniela .
DAIRY SCIENCE & TECHNOLOGY, 2011, 91 (04) :457-470
[7]   Molecular diversity of Leuconostoc mesenteroides and Leuconostoc citreum isolated from traditional French cheeses as revealed by RAPD fingerprinting, 16S rDNA sequencing and 16S rDNA fragment amplification [J].
Cibik, R ;
Lepage, E ;
Tailliez, P .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2000, 23 (02) :267-278
[8]   METABOLISM OF LEUCONOSTOC BACTERIA [J].
COGAN, TM ;
JORDAN, KN .
JOURNAL OF DAIRY SCIENCE, 1994, 77 (09) :2704-2717
[9]  
Dimic G., 2006, Apteff, V37, P3, DOI [10.2298/APT0637003D, DOI 10.2298/APT0637003D]
[10]   Reclassification of the genus Leuconostoc and proposals of Fructobacillus fructosus gen. nov., comb. nov., Fructobacillus durionis comb. nov., Fructobacillus ficulneus comb. nov and Fructobacillus pseudoficulneus comb. nov. [J].
Endo, Akihito ;
Okada, Sanae .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008, 58 :2195-2205