Insoluble but enzymatically active α-amylase from Bacillus licheniformis

被引：8
|
作者：
Rashid, Naeem [1 ]
Farooq, Alia [1 ,2 ]
Ikram-ul-Haq [2 ]
Akhtar, Muhammad [1 ]
机构：
[1] Univ Punjab, Sch Biol Sci, Lahore 54590, Pakistan
[2] Govt Coll Univ, Inst Ind Biotechnol, Lahore 54000, Pakistan
来源：
BIOLOGIA | 2009年 / 64卷 / 04期
关键词：
Bacillus licheniformis; cloning; alpha-amylase; inclusion bodies; STARCH-BINDING DOMAIN; ESCHERICHIA-COLI; SIGNAL PEPTIDE; T7; PROMOTER; EXPRESSION; EVOLUTION; SECRETION; AMYLOLIQUEFACIENS; PURIFICATION; SUBTILIS;
D O I：
10.2478/s11756-009-0132-5
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The gene encoding thermostable alpha-amylase from Bacillus licheniformis consisting of 483 amino acid residues (mature protein) was cloned and expressed in Escherichia coli under the control of T7 promoter. The analysis of the soluble and insoluble fractions after lyzing the host cells revealed that recombinant alpha-amylase was produced in insoluble aggregates. Despite being produced in the insoluble aggregates the recombinant enzyme was highly active with a specific activity of 408 U/mg.
引用
收藏
页码：660 / 663
页数：4
相关论文
共 50 条
  • [41] Enhancement of acid stability of alpha amylase from Bacillus licheniformis by error-prone PCR
    Xu, Yanjing
    Liu, Yihan
    Fan, Shuai
    Lu, Fuping
    ADVANCED TECHNOLOGIES IN MANUFACTURING, ENGINEERING AND MATERIALS, PTS 1-3, 2013, 774-776 : 664 - 669
  • [42] EFFECT OF TEMPERATURE ON SOME CHARACTERISTICS OF THE THERMOSTABLE ALPHA-AMYLASE FROM BACILLUS-LICHENIFORMIS
    DOBREVA, E
    IVANOVA, V
    EMANUILOVA, E
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1994, 10 (05): : 547 - 550
  • [43] High Level Production of Extracellular α-Amylase from Bacillus licheniformis ATCC 12759 in Submerged Fermentation
    Akcan, Nurullah
    ROMANIAN BIOTECHNOLOGICAL LETTERS, 2011, 16 (06): : 6833 - 6840
  • [44] Characterization and application of alkaline α-amylase from Bacillus licheniformis MTCC1483 as a detergent additive
    Dahiya, P.
    Rathi, B.
    INTERNATIONAL FOOD RESEARCH JOURNAL, 2015, 22 (03): : 1293 - 1297
  • [45] Production optimization, purification and characterization of α-amylase from thermophilic Bacillus licheniformis TSI-14
    Shukla, Rushit J.
    Singh, Satya P.
    STARCH-STARKE, 2015, 67 (7-8): : 629 - 639
  • [46] Enhanced production of heterologous proteins by Bacillus licheniformis with defective d-alanylation of lipoteichoic acid
    Chen, Yaozhong
    Cai, Dongbo
    He, Penghui
    Mo, Fei
    Zhang, Qing
    Ma, Xin
    Chen, Shouwen
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2018, 34 (09):
  • [47] Minimization and optimization of α-amylase terminator for heterologous protein production in Bacillus licheniformis
    Rao, Yi
    Yang, Jingyao
    Wang, Jiaqi
    Yang, Xinyuan
    Zhang, Mengxi
    Zhan, Yangyang
    Ma, Xin
    Cai, Dongbo
    Wang, Zhangqian
    Chen, Shouwen
    BIORESOURCES AND BIOPROCESSING, 2022, 9 (01)
  • [48] Cloning, Functional Expression and Characterization of an Alkaline Protease from Bacillus licheniformis
    Ritu Sareen
    Uwe T. Bornscheuer
    Prashant Mishra
    Biotechnology Letters, 2005, 27 : 1901 - 1907
  • [49] Cloning, functional expression and characterization of an alkaline protease from Bacillus licheniformis
    Sareen, R
    Bornscheuer, UT
    Mishra, P
    BIOTECHNOLOGY LETTERS, 2005, 27 (23-24) : 1901 - 1907
  • [50] Development of an Inosine Hyperproducer from Bacillus licheniformis by Systems Metabolic Engineering
    Zhou, Menglin
    Li, Yi
    Cai, Youhua
    Sun, Yaqi
    Chen, Wu
    Wang, Jin
    Shen, Feng
    Zhan, Yangyang
    Ying, Jun
    Chen, Shouwen
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023, 71 (50) : 20210 - 20221
12345