Endophytic fungi found in association with Smallanthus sonchifolius (Asteraceae) as resourceful producers of cytotoxic bioactive natural products

被引：32
作者：
Gallo, Margareth B. C. [1 ]
Chagas, Fernanda O. [1 ]
Almeida, Marilia O. [1 ]
Macedo, Claudia C. [1 ]
Cavalcanti, Bruno C. [2 ]
Barros, Francisco W. A. [2 ]
de Moraes, Manoel O. [2 ]
Costa-Lotufo, Leticia V. [2 ]
Pessoa, Claudia [2 ]
Bastos, Jairo K. [1 ]
Pupo, Monica T. [1 ]
机构：
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, Brazil
[2] Univ Fed Ceara, Dept Fisiol & Farmacol, Fortaleza, Ceara, Brazil
来源：
JOURNAL OF BASIC MICROBIOLOGY | 2009年 / 49卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
Nigrospora sphaerica; Phoma betae; Yacon; Aphidicolin; Isocoumarin; LEAF EXTRACTS; YACON ROOTS; METABOLITES; BIOTRANSFORMATION; COMMUNITIES; ISOCOUMARIN; NIGROSPORA; TOXICITY; GROWTH; ACID;
D O I：
10.1002/jobm.200800093
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Smallanthus sonchifolius is a traditional Andean plant which has been cultured mainly in Brazil, Japan and New Zealand due to its medicinal properties. A study of the endophytic fungi associated to the plant was carried out in order to characterize new cytotoxic agents. Thirty two fungal strains were isolated and submitted to cultivation and extraction producing 186 extracts. Of these, 12% displayed moderate to high cytotoxic activities and were considered promising anticancer compound sources. The ethyl acetate fractions of Nigrospora sphaerica and Phoma betae liquid fermentations contained the synergistic compounds 8-hydroxy-6-methoxy-3-methylisocoumarin and (22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one which are potential compounds for drug discovery. Another isolated compound, pimara-7,15-dien-3-beta-ol diterpene is being characterized for the first time through a detailed spectroscopic analysis including GC/MS, homo- and hetero-nuclear correlated NMR experiments (HMQC, HMBC, COSY and NOEdiff) along with its optical rotation.
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页码：142 / 151
页数：10
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