Evaluation of in vivo conception after testicular stem cell transplantation in a mouse model shows altered post-implantation development

被引：15

作者：

Goossens, Ellen ^{[1
]}

Frederickx, Veerle ^{[1
]}

de Block, Gert ^{[1
]}

van Steirteghem, Andre ^{[1
]}

Tournaye, Herman ^{[1
]}

机构：

[1] Free Univ Brussels, Fac Med & Pharm, Res Lab Reprod & Genet, B-1090 Brussels, Belgium

来源：

HUMAN REPRODUCTION | 2006年 / 21卷 / 08期

关键词：

fetus; offspring; spermatogenesis; testis; transplantation;

D O I：

10.1093/humrep/del105

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

BACKGROUND: Apart from research applications, testicular stem cell transplantation (TSCT) may one day also have valuable clinical applications. Therefore, it is important to investigate whether this technique is a safe method to have progeny. This controlled study aims at evaluating the fetuses and the live born offspring obtained after TSCT in male mice. METHODS: Male mice were mated with wild-type (WT) females after TSCT to produce offspring. First, fetuses were evaluated on the 17th gestational day. The length, weight and morphological age were compared to those of control mouse fetuses. The live born offspring were then investigated for their reproductive potential over three generations. RESULTS: The litter sizes after TSCT were decreased compared to controls. Fetuses showed developmental retardation of a quarter of a day, but no major external abnormalities were observed. The live born pups were able to produce normal litter sizes, at least until the third generation. CONCLUSIONS: Transplanted animals are able to reproduce naturally. Although litter sizes are lower and development is retarded, no major morphological or procreative abnormalities were observed.

引用

页码：2057 / 2060

页数：4

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