The present experiment was designed to stuck, progressive changes ill the physiological responses of broiler chickens (Gallus gallus) exposed to different durations of acute heat stress. Male broiler chickens (n = 8) at 16 clays of age. were exposed to acute heat stress of 34 degrees C for 6, 12 or 18 h while control chickens were kept at 25 degrees C. Exposure to different durations of heat stress resulted ill significant decreases in the levels of blood HCO3 and pCO(2), while pH and pO(2) were not changed. Hemoglobin, hematocrit and Na+ values were reduced after 6 h while no such change was observed after 12 or 18 h of heat stress. In contrast, K+ levels were lowered by 6 or 12 h of heat stress acid no differences in blood calcium levels were observed between the control and heat-stressed groups. In conclusion, there is evidence that exposure of broiler chickens to different durations of acute heat stress resulted in distinct time-dependent physiological responses. Data set from this experimental model could provide an overview of physiological state in the typical acute heat-stressed birds.