Multiphoton microscopy using frequency-doubled compact femtosecond erbium-doped fiber laser

被引：0

作者：

Huang, Lin ^{[1
]}

Chong, Shau Poh ^{[1
]}

Mills, Arthur ^{[2
]}

Jones, David ^{[2
]}

Tang, Shuo ^{[1
]}

机构：

[1] Univ British Columbia, Dept Elect & Comp Engn, Vancouver, BC V6T 1Z4, Canada

[2] Univ British Columbia, Dept Phys & Astron, Vancouver, BC V6T 1Z1, Canada

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIV | 2014年 / 8948卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

nonlinear microscopy; ultrafast lasers; medical and biological imaging; FLUORESCENCE; ENDOMICROSCOPY;

D O I：

10.1117/12.2036052

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We report on the development of a compact multiphoton microscopy (MPM) system based on a frequency-doubled, femtosecond erbium-doped fiber laser source at 1.58 mu m. By use of periodically poled MgO:LiNbO3, frequency-doubled pulses at 790 nm with average power of 75 mW and pulse width of 130 fs are applied as the excitation source. The fiber laser is optimized for its parameters along with the dispersive properties of the delivery fiber such that the MPM signal is maximized at the sample location. Micro-electro-mechanical system (MEMS) scanner, miniature objective, and multimode fiber are further used to make the MPM system compact. MPM images are obtained from unstained biological samples. The MPM system with a compact, portable, low-cost fiber laser has a great potential to transform the bench-top MPM system to a portable system for in vivo MPM imaging.

引用

页数：6