Generation of duck hepatitis B virus polymerase mutants through site-directed mutagenesis which demonstrate resistance to lamivudine [(-)-beta-L-2',3'-dideoxy-3'-thiacytidine] in vitro

被引：60
作者：
Fischer, KP
Tyrrell, DLJ
机构：
[1] UNIV ALBERTA,DEPT MED MICROBIOL & IMMUNOL,EDMONTON,AB T6G 2H7,CANADA
[2] UNIV ALBERTA,GLAXOWELLCOME HERITAGE RES INST,EDMONTON,AB T6G 2H7,CANADA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1996年 / 40卷 / 08期
关键词：
D O I：
10.1128/AAC.40.8.1957
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Hepatitis B virus replication is very sensitive to lamivudine. A single amino acid change in the human immunodeficiency virus reverse transcriptase is responsible for high-level resistance to this compound. Duck hepatitis B virus mutants were created bearing the analogous amino acid change in the duck hepatitis B virus polymerase. Viral DNA production was reduced 92% for the wild-type virus at 2 mu g of lamivudine per ml, while the mutants required 40 mu g of lamivudine per ml to inhibit replication by greater than 80%.
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页码：1957 / 1960
页数：4
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