Fast in vivo multiphoton light-sheet microscopy with optimal pulse frequency

被引：18
作者：
Maioli, Vincent [1 ]
Boniface, Antoine [1 ]
Mahou, Pierre [1 ]
Ortas, Julia Ferrer [1 ]
Abdeladim, Lamiae [1 ]
Beaurepaire, Emmanuel [1 ]
Supatto, Willy [1 ]
机构：
[1] Inst Polytech Paris, Lab Opt & Biosci, INSERM, CNRS,Ecole Polytech, F-91128 Palaiseau, France
来源：
BIOMEDICAL OPTICS EXPRESS | 2020年 / 11卷 / 10期
关键词：
HIGH-SPEED; 2-PHOTON; DEEP; PHOTODAMAGE; DYNAMICS; EMBRYOS;
D O I：
10.1364/BOE.400113
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Improving the imaging speed of multiphoton microscopy is an active research field. Among recent strategies, light-sheet illumination holds distinctive advantages for achieving fast imaging in vivo. However, photoperturbation in multiphoton light-sheet microscopy remains poorly investigated. We show here that the heart beat rate of zebrafish embryos is a sensitive probe of linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency and wavelength, we derive guidelines to find the best balance between signal and photoperturbation. We then demonstrate one order-of-magnitude signal enhancement over previous implementations by optimizing the laser pulse frequency. These results open new Opportunities for fast live tissue imaging. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
引用
收藏
页码：6012 / 6026
页数：15
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