A technique for measuring xylem hydraulic conductance under high negative pressures

被引：152

作者：

Cochard, H ^{[1
]}

机构：

[1] Univ Clermont Ferrand, INRA, UMR PIAF, F-63039 Clermont Ferrand, France

来源：

PLANT CELL AND ENVIRONMENT | 2002年 / 25卷 / 06期

关键词：

Laurus nobilis L; cavitation; centrifugation; embolism; water relations;

D O I：

10.1046/j.1365-3040.2002.00863.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A technique for measuring hydraulic conductances of excised xylem segments exposed to high negative pressures is described. A centrifugal force is used to generate negative pressures (P) in the sample and to create a positive hydrostatic pressure difference (DeltaP) between its two ends. DeltaP forces water through the sample at a flow rate (F) determined optically during centrifugation. The sample hydraulic conductance k is derived from F and DeltaP . The sample vulnerability curve is given by the dependence of k on P . Results for Cedrus atlantica Manetti and Laurus nobilis L. shoots are given. The technique is appropriate for the analysis of xylem refilling under negative pressure.

引用

页码：815 / 819

页数：5

共 18 条

[1] Use of centrifugal force in the study of xylem cavitation [J].

Alder, NN ;

Pockman, WT ;

Sperry, JS ;

Nuismer, S .

JOURNAL OF EXPERIMENTAL BOTANY, 1997, 48 (308) :665-674

[2] Transporting water in plants [J].

Canny, MJ .

AMERICAN SCIENTIST, 1998, 86 (02) :152-159

[3] Cryo-scanning electron microscopy observations of vessel content during transpiration in walnut petioles.: Facts or artifacts? [J].

Cochard, H ;

Bodet, C ;

Améglio, T ;

Cruiziat, P .

PLANT PHYSIOLOGY, 2000, 124 (03) :1191-1202

[4] USE OF POSITIVE PRESSURES TO ESTABLISH VULNERABILITY CURVES - FURTHER SUPPORT FOR THE AIR-SEEDING HYPOTHESIS AND IMPLICATIONS FOR PRESSURE-VOLUME ANALYSIS [J].

COCHARD, H ;

CRUIZIAT, P ;

TYREE, MT .

PLANT PHYSIOLOGY, 1992, 100 (01) :205-209

[5] Unraveling the effects of plant hydraulics on stomatal closure during water stress in walnut [J].

Cochard, H ;

Coll, L ;

Le Roux, X ;

Améglio, T .

PLANT PHYSIOLOGY, 2002, 128 (01) :282-290

[6] The cohesion theory debate continues -: Response by Herve!Cochard, Thierry!Ameglio and Pierre!Cruiziat [J].

Cochard, H ;

Améglio, T ;

Cruiziat, P .

TRENDS IN PLANT SCIENCE, 2001, 6 (10) :456-456

[7] Vessel content debate revisited [J].

Cochard, H ;

Améglio, T ;

Cruiziat, P .

TRENDS IN PLANT SCIENCE, 2001, 6 (01) :13-13

[8] VULNERABILITY OF SEVERAL CONIFERS TO AIR-EMBOLISM [J].

COCHARD, H .

TREE PHYSIOLOGY, 1992, 11 (01) :73-83

[9]

CRUIZIAT P, 2002, IN PRESS ANN FOREST

[10] Embolism repair and xylem tension: Do we need a miracle? [J].

Holbrook, NM ;

Zwieniecki, MA .

PLANT PHYSIOLOGY, 1999, 120 (01) :7-10

← 1 2 →