Direct coupling of expanded bed adsorption with a downstream purification step

被引:8
|
作者
Beck, JT [1 ]
Williamson, B [1 ]
Tipton, B [1 ]
机构
[1] Amgen Inc, Purificat Proc Dev, Thousand Oaks, CA 91320 USA
关键词
D O I
10.1023/A:1008058602398
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In the course of developing a cost-effective, scaleable process for the purification of a recombinant protein from Chinese hamster ovary (CHO) suspension cell culture, we investigated direct capture of this molecule using expanded bed adsorption (EBA). EBA combines clarification, purification, and concentration of the product into a single step. The unclarified bioreactor material was directly applied to a STREAMLINE 25 column containing an affinity STREAMLINE adsorbent. This work focused on simplifying the EBA operations and minimizing the overall processing time by running the EBA column unidirectionally, eluting in the expanded bed mode, and coupling the EBA column directly with ion exchange or hydrophobic interaction chromatography. Unidirectional EBA was clearly a simpler unit operation and did not require the use of specialized equipment. The increase in the elution pool volume was insignificant, especially when the EBA column was eluted directly onto the downstream column. Scale-down was simple and could be automated. Coupling of unidirectional EBA with a downstream purification step reduced processing time, equipment requirements and cost.
引用
收藏
页码:201 / 207
页数:7
相关论文
共 50 条
  • [41] Recovery of extracellular inulinase by expanded bed adsorption
    Pessoa, A
    Hartmann, R
    Vitolo, M
    Hustedt, H
    JOURNAL OF BIOTECHNOLOGY, 1996, 51 (01) : 89 - 95
  • [42] Biochemical engineering aspects of expanded bed adsorption
    Hubbuch, J
    Thömmes, J
    Kula, MR
    TECHNOLOGY TRANSFER IN BIOTECHNOLOGY: FROM LAB TO INDUSTRY TO PRODUCTION, 2005, 92 : 101 - 123
  • [43] Efficiency of expanded bed adsorption processes.
    Hjorth, RA
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2003, 225 : U206 - U206
  • [44] Recovery and purification of chitosanase produced by Bacillus cereus using expanded bed adsorption and central composite design
    de Araujo, Nathalia Kelly
    Pimentel, Vanessa Carvalho
    Portela da Silva, Nayane Macedo
    de Araujo Padilha, Carlos Eduardo
    de Macedo, Gorete Ribeiro
    dos Santos, Everaldo Silvino
    JOURNAL OF SEPARATION SCIENCE, 2016, 39 (04) : 709 - 716
  • [45] Intensified process for the purification of an enzyme from inclusion bodies using integrated expanded bed adsorption and refolding
    Hutchinson, Matthew H.
    Chase, Howard A.
    BIOTECHNOLOGY PROGRESS, 2006, 22 (04) : 1187 - 1193
  • [46] PURIFICATION OF PROTEINS BY ADSORPTION CHROMATOGRAPHY IN EXPANDED BEDS
    CHASE, HA
    TRENDS IN BIOTECHNOLOGY, 1994, 12 (08) : 296 - 303
  • [47] Single-step recovery of ephedrine hydrochloride from raw materials using expanded bed adsorption
    Chen, WD
    Wang, YD
    Zha, LH
    Ma, GH
    Su, ZG
    BIOTECHNOLOGY LETTERS, 2004, 26 (15) : 1233 - 1236
  • [48] Single-step recovery of ephedrine hydrochloride from raw materials using expanded bed adsorption
    Wei-Dong Chen
    Yan-Dong Wang
    Li-hang Zha
    Guang-Hui Ma
    Zhi-Guo Su
    Biotechnology Letters, 2004, 26 : 1233 - 1236
  • [49] Purification of lactoferrin by expanded-bed column chromatography
    Shiozawa, M
    Okabe, H
    Nakagawa, Y
    Morita, H
    Uchida, T
    KAGAKU KOGAKU RONBUNSHU, 2001, 27 (02) : 145 - 148
  • [50] Purification of adenoviral vectors using expanded bed chromatography
    Peixoto, C
    Ferreira, TB
    Carrondo, MJT
    Cruz, PE
    Alves, PM
    JOURNAL OF VIROLOGICAL METHODS, 2006, 132 (1-2) : 121 - 126