A Tyrosine Aminomutase from Rice (Oryza sativa) lsomerizes (S)-α- to (R)-β-Tyrosine with Unique High Enantioselectivity and Retention of Configuration

被引：15

作者：

Walter, Tyler ^{[1
]}

King, Zayna ^{[3
]}

Walker, Kevin D. ^{[1
,2
]}

机构：

[1] Michigan State Univ, Dept Chem, E Lansing, MI 48824 USA

[2] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA

[3] CUNY Medgar Evers Coll, Brooklyn, NY 11225 USA

来源：

BIOCHEMISTRY | 2016年 / 55卷 / 01期

关键词：

PHENYLALANINE AMINOMUTASE; BIOSYNTHESIS; MECHANISM; STEREOCHEMISTRY; PATHWAY;

D O I：

10.1021/acs.biochem.5b01331

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recently discovered 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO)-dependent tyrosine aminomutase (OsTAM) from rice [Yan, J., et al. (2015) Plant Cell 27, 1265] converts (S)-alpha-tyrosine to a mixture of (R)- and (S)-beta-tyrosines, with high (94%) enantiomeric excess, which does not change with pH, like it does for two bacterial TAMs. The K-M of 490 mu M and the k(cat) of 0.005 s(-1) are similar for other TAM enzymes. OsTAM is unique and also catalyzes (R)-beta- from (S)-alpha-phenylalanine. OsTAM principally retains the configuration at the reactive C-alpha and C-beta centers during catalysis much like the phenylalanine aminomutase on the Taxol biosynthetic pathway in Taxus plants.

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页码：1 / 4

页数：4

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