COMPARISON OF THE EFFECTS OF SOIL TREATMENT WITH NaF AND KF ON ANTIOXIDANT ENZYMES IN WINTER WHEAT (TRITICUM AESTIVUM L.) SEEDLINGS

This paper assesses the impact of the treatment of soil with NaF and KF on activity of antioxidant enzymes involved in the Halliwell-Asada cycle: glutathione reductase (GR), dehydroascorbate reductase (DHAR), ascorbate peroxidase (APX), and antioxidant enzymes that decompose hydrogen peroxide: catalase (CAT), and guaiacol peroxidase (POD) in winter wheat cv. Skagen seedlings. The fluoride was added to loamy sand at concentrations of 10 mmol/kg DM soil and 30 mmol/kg DM soil. This study demonstrated that soil treatment with fluorine salts caused mainly increase in the activity of the studied enzymes. The highest changes occurred in DHAR and POD activity. The influence of NaF was typically higher than that of KF, while based on eta(2) it was determined that the activity of GR and POD was mostly shaped by the salt concentration added to soil, and those of DHAR, APX, and CAT by the time of measurement after sowing. The conducted principal component analysis demonstrated that the activity of all determined enzymes was negatively correlated with the first principal component.