ANALYSIS OF ROLE OF OXIDATIVE STRESS IN THE DEVELOPMENT OF DIABETES MELLITUS AMONG LOCAL POPULATION OF PAKISTAN

Introduction: Oxidative stress is caused by an unfavorable balance between reactive oxygen species (ROS) and antioxidant defenses. ROS are generated during normal cellular metabolism, as a result of the influence of various environmental factors, as well as during pathological processes. Objectives: The main objective of our study is to find the level of oxidative stress and their role in the cause of diabetes. Material and Methods: The study was conducted at Nishtar Hospital, Multan during Jan 2018 to March 2018. This study is based on the local population of Pakistan, which shows the stress level in Pakistani environment. 5.0 ml blood sample was taken from vein. Blood was further processed for the estimation of GSH, Catalases, SOD, MDA, Neuraminidase and Sialic acid. Commercially available enzymatic kits of Randox were used. Results: Level of antioxidant and oxidative stress is increasing in diabetic patients because cell become destroyed. GSH is important non - enzymatic antioxidant which helps in scavenging of free radical mechanism. According to data the levels of GSH become decreases in diabetic patients. The data pertaining in the table shows that levels of sialic acid become increases in patients. The level becomes increases in all cases. As the value in this case is 3.48 +/- 0.65. According to our data MDA is considered to be an important antioxidant and serum stress biomarker in case of diabetic patients. Conclusion: It is concluded that level of antioxidants in our body plays an important role. It is obvious from the presented data that a relation exists between hyperglycaemia, oxidative stress, cellular and endothelial dysfunction.