Thermostable xylanases produced at 37 degrees C and 45 degrees C by a thermotolerant Aspergillus strain

被引:25
作者
Castro, LPM [1 ]
TrejoAguilar, BA [1 ]
Osorio, GA [1 ]
机构
[1] UNIV NACL AUTONOMA MEXICO, FAC CHEM, DEPT FOOD SCI & BIOTECHNOL, CONJ E, MEXICO CITY 04510, DF, MEXICO
关键词
xylanolytic system; Aspergillus FP-470; temperature stress;
D O I
10.1016/S0378-1097(96)00460-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Differences between xylanases produced by Aspergillus FP-470 growing under normal (37 degrees C) and temperature stress conditions (45 degrees C) were evaluated. Fungal growth at 45 degrees C was seriously affected. However, the xylanase specific activity was 2.5 times higher than that produced at 37 degrees C. Optimum pH and temperature were 6.5, 80 degrees C and 4.3, 50 degrees C for the xylanases produced at 37 degrees C and 45 degrees C, respectively. Electrophoresis showed that a more complex xylanolytic system was produced at 37 degrees C. Stress induced by temperature produced a decrease in the number of extracellular xylanases released by fungi, but increased the production of some xylanases, possibly essential for growth under stress conditions.
引用
收藏
页码:97 / 102
页数:6
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